3x DYKDDDDK Tag;
3x DYKDDDDK Tag 是研究抗体 (Abs) 不可用的蛋白质的功能和定位的有用工具。通常，它也以 3X 标志形式 (3x DYKDDDDK Tag TFA) 用于纯化低丰度蛋白。
3x DYKDDDDK Tag Chemical Structure
CAS No. : 921765-76-6
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3x DYKDDDDK Tag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance .
HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS).
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
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