Mad1 (6-21) (TFA)

Mad1 (6-21) (TFA);

Mad1 (6-21) TFA 是 Mad1 蛋白的 6-21 片段。Mad1 (6-21) TFA 与哺乳动物 Sin3A PAH2 结合,Kd 为 ~29 nM。

Mad1 (6-21) (TFA)amp;;

Mad1 (6-21) (TFA) Chemical Structure

规格 是否有货
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生物活性

Mad1 (6-21) TFA is the 6-21 fragment of Mad1 protein. Mad1 (6-21) TFA binds to mammalian Sin3A PAH2 with a Kd of ~29 nM[1].

体外研究
(In Vitro)

The PAH2 domain of mSin3A adopts a left-handed, up-and-down, four-helix bundle structure with residues in all four helices as well as in the turn regions defining a compact structural domain with an extensive hydrophobic core. Helices α1 and α2 form a deep hydrophobic pocket, which constitutes the primary interaction surface for the Mad1 (6-21) peptide. The Mad1 (6-21) forms an amphipathic α helix in the complex and interacts with PAH2 mainly through the apolar surface of the helix[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2080.31

Formula

C86H141F3N24O28S2

Sequence Shortening

RMNIQMLLEAADYLER

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. K Brubaker, et al. Solution structure of the interacting domains of the Mad-Sin3 complex: implications for recruitment of a chromatin-modifying complex. Cell. 2000 Nov 10;103(4):655-65.

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