TAT peptide

发表于由

TAT peptide;

TAT peptide 是一种可渗透细胞的肽 (GRKKRRQRRRPQ)，来自HIV-1反转录激活因子 (Tat)。

TAT peptideamp;;

TAT peptide Chemical Structure

规格 价格 是否有货
1 mg ￥1200 询问价格 货期
5 mg ￥4800 询问价格 货期
10 mg ￥8100 询问价格 货期

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TAT peptide 的其他形式现货产品:

TAT peptide TFA

生物活性

TAT peptide is a cell penetrating peptide (GRKKRRQRRRPQ) derived from the trans-activating transcriptional activator (Tat) from HIV-1[1][2].

体外研究
(In Vitro)

TAT peptide is a cell penetrating peptide (GRKKRRQRRRPQ) derived from the trans-activating transcriptional activator (Tat) from HIV-1[1]. TAT peptide (GRKKRRQRRRPQ) functionalized hybrid nanoparticles are also studied due to their combined magnetic enrichment and optical detection for cell separation and rapid cell labelling. A cell viability assay reveals good biocompatibility of these hybrid nanoparticles[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1621.91

Formula

C65H124N34O15
Sequence

Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Gln
Sequence Shortening

GRKKRRQRRRPQ
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Orzáez M, et al. Intrinsic caspase-8 activation mediates sensitization of erlotinib-resistant tumor cells toerlotinib/cell-cycle inhibitors combination treatment. Cell Death Dis. 2012 Oct 25;3:e415.

    [2]. Lou L, et al. Functionalized magnetic-fluorescent hybrid nanoparticles for cell labelling. Nanoscale. 2011 May;3(5):2315-23.