Recombinant Proteinase K(Synonyms: 重组蛋白酶 K)

Recombinant Proteinase K;(Synonyms: 重组蛋白酶 K)

Recombinant Proteinase K 是一种丝氨酸蛋白酶 (serine protease),它能裂解脂肪族和芳香族氨基酸的羧基端肽键。Recombinant Proteinase K 可用于消化蛋白质和清除核酸制剂中的污染。

Recombinant Proteinase Kamp;;(Synonyms: 重组蛋白酶 K)

Recombinant Proteinase K Chemical Structure

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Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations[1].

(In Vitro)

Proteinase K digestion[1]
1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.
2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 µl aliquot to get the final concentrations of 2, 4 and 8 µg/ml. Reactions are carried out at 37°C for 30 minutes.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.


重组蛋白酶 K


Room temperature in continental US; may vary elsewhere.

Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
  • [1]. Sabine Gauczynski, et al. Recombinant human prion protein mutants huPrP D178N/M129 (FFI) and huPrP+9OR (fCJD) reveal proteinase K resistance. J Cell Sci. 2002 Nov 1;115(Pt 21):4025-36.