Recombinant Proteinase K;(Synonyms: 重组蛋白酶 K)
Recombinant Proteinase K 是一种丝氨酸蛋白酶 (serine protease)，它能裂解脂肪族和芳香族氨基酸的羧基端肽键。Recombinant Proteinase K 可用于消化蛋白质和清除核酸制剂中的污染。
Recombinant Proteinase K Chemical Structure
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Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations.
Proteinase K digestion1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 µl aliquot to get the final concentrations of 2, 4 and 8 µg/ml. Reactions are carried out at 37°C for 30 minutes.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Room temperature in continental US; may vary elsewhere.