T7 Tag Peptide(Synonyms: T7标签肽)

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T7 Tag Peptide;(Synonyms: T7标签肽)

T7 Tag Peptide 是一个蛋白标签，由 T7 噬菌体衣壳蛋白 gp 10 N-端的 11个残基组成。T7 Tag Peptide 可用于不同的免疫检测以及亲和纯化。

T7 Tag Peptideamp;;(Synonyms: T7标签肽)

T7 Tag Peptide Chemical Structure

CAS No. : 245445-88-9

规格 价格 是否有货
1 mg ￥600 询问价格 货期
5 mg ￥1200 询问价格 货期
10 mg ￥2100 询问价格 货期

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T7 Tag Peptide 的其他形式现货产品:

T7 Tag Peptide TFA

生物活性

T7 Tag Peptide is a protein tag derived from the N-terminal 11 residues of the major T7 capsid protein, gp 10. T7 Tag Peptide can be used in different immunoassays as well as affinity purification[1].

分子量

1098.27

Formula

C41H71N13O16S3
CAS 号

245445-88-9
Sequence

Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly
Sequence Shortening

MASMTGGQQMG
中文名称

T7标签肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Tsutomu Tanaka, et al. A Peptidyl Linker for Protein Cross-Linking Catalyzed by Microbial Transglutaminase. The Society of Chemical Engineers, Japan. Session ID : 3J-11.