EGF Receptor Substrate 2 Phospho-Tyr5(Synonyms: 表皮生长因子受体底物2 (phospho-tyr5))

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EGF Receptor Substrate 2 Phospho-Tyr5;(Synonyms: 表皮生长因子受体底物2 (phospho-tyr5)) 纯度: 98.70%

EGF Receptor Substrate 2 (Phospho-Tyr5) 是一种活性肽，它来源于表皮生长因子受体自身磷酸化位点 (酪氨酸992)。

EGF Receptor Substrate 2 Phospho-Tyr5amp;;(Synonyms: 表皮生长因子受体底物2 (phospho-tyr5))

EGF Receptor Substrate 2 Phospho-Tyr5 Chemical Structure

规格 价格 是否有货 数量
1 mg ￥900 In-stock
5 mg ￥2600 In-stock
10 mg ￥4600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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EGF Receptor Substrate 2 Phospho-Tyr5 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

EGF Receptor Substrate 2 (Phospho-Tyr5) is a biologically active peptide derived from an autophosphorylation site (Tyr992) of epidermal growth factor receptor (EGFR).

体外研究
(In Vitro)

DADEpYLIPQQG is a generalized PTPase substrate. Both CAV GST-VP2 and TLMV GST-ORF2 are shown to have PTPase activity using both ENDpYINASL and DADEpYLIPQQG. Steady state activities for the reaction of 5 μg of CAV GST-VP2 with ENDpYINASL and DADEpYLIPQQG are 100 and 208%, respectively, of those seen for 2 units of TC-PTP[1]. In the context of DADEpYLIPQQG, the minimal sizes recognized by PTPα are either ADEpYLI or DADEpY-NH2. The kcat/Km value for the parent peptide DADEpYLIPQQG (EGFR988-998) is 1090-fold higher than Tyr(P)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1328.28

Formula

C54H82N13O24
Sequence

H-Asp-Ala-Asp-Glu-{pTyr}-Leu-Ile-Pro-Gln-Gln-Gly-OH
Sequence Shortening

HDADE-{pTyr}-LIPQQG-OH
中文名称

表皮生长因子受体底物2 (phospho-tyr5)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Peters MA, et al. Chicken anemia virus VP2 is a novel dual specificity protein phosphatase. J Biol Chem. 2002 Oct 18;277(42):39566-73. Epub 2002 Jul 31.

    [2]. Wu L, et al. Comparative kinetic analysis and substrate specificity of the tandem catalytic domains of the receptor-like protein-tyrosine phosphatase alpha. J Biol Chem. 1997 Mar 14;272(11):6994-7002.
Kinase Assay
[1]

Briefly, the assays are performed in 50 μL volumes in a microtiter plate using the generalized PTPase substrates ENDpYINASL and DADEpYLIPQQG (where pY represents phosphotyrosine) and assay buffer containing 25 mM Tris-HCl (pH 7.4), 50 mM NaCl, 2 mM EDTA, 5 mM dithiothreitol, 0.01% Brij 35, and 1 mg of bovine serum albumin/mL. The reactions are started by the addition of 5 μg of either CAV GST-VP2, GST, CAV GST-VP2 containing the C95S mutation, or TLMV GST-ORF2 or 2 units of the positive control T cell protein-tyrosine phosphatase (TC-PTP) in assay buffer. Control reactions are assayed with either ENDpYINASL substrate alone, DADEpYLIPQQG substrate alone, CAV GST-VP2 without substrate, CAV GST-VP2 containing the C95S mutation without substrate, TLMV GST-ORF2 without substrate, TC-PTP without substrate, or assay buffer with neither enzyme nor substrate. A phosphate standard curve is derived using a supplied phosphate standard. The reactions are terminated by the addition of the malachite green detection reagent.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Peters MA, et al. Chicken anemia virus VP2 is a novel dual specificity protein phosphatase. J Biol Chem. 2002 Oct 18;277(42):39566-73. Epub 2002 Jul 31.

    [2]. Wu L, et al. Comparative kinetic analysis and substrate specificity of the tandem catalytic domains of the receptor-like protein-tyrosine phosphatase alpha. J Biol Chem. 1997 Mar 14;272(11):6994-7002.