Indolicidin

Indolicidin;

Indolicidin是从牛嗜中性粒细胞的细胞质颗粒中纯化的有效的抗微生物肽。

Indolicidinamp;;

Indolicidin Chemical Structure

CAS No. : 140896-21-5

规格 价格 是否有货 数量
500 μg ¥1600 In-stock
1 mg ¥2600 In-stock
5 mg ¥7800 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Indolicidin is a potent antimicrobial peptide purified from the cytoplasmic granules of bovine neutrophils.

体外研究
(In Vitro)

Indolicidin is comprised of 13 amino acids, 5 of which are tryptophan residues, and the carboxylterminal arginine is carboxamidated. Indolicidin has the highest tryptophan content of any known protein. The multiple tryptophan residues may play an important role in the function of this unique antibiotic peptide. Indolicidin is a tridecapeptide amide which possesses in vitro bactericidal activities comparable with the most active of the defensin or bactenecin peptides[1]. Indolicidin binds purified surface lipopolysaccharide with high affinity and permeabilized the outer membrane of Escherichia coli to the small hydrophobic molecule 1-N-phenylnapthylamine (Mr 200), results consistent with indolicidin crossing the outer membrane via the self-promoted uptake pathway. The methyl esterification of indolicidin’s carboxyl terminus increases its activity for Gram-negative and Gram-positive bacteria. In Gram-negative bacteria this is associated with an increased binding to lipopolysaccharide and increased permeabilization of the outer membrane. The cytoplasmic membrane is the site of action of indolicidin as assayed in Escherichia coli by the unmasking of cytoplasmic beta-galactosidase due to membrane permeabilization[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1906.28

Formula

C100H132N26O13

CAS 号

140896-21-5

Sequence

Ile-Leu-Pro-Trp-Lys-Trp-Pro-Trp-Trp-Pro-Trp-Arg-Arg-NH2

Sequence Shortening

ILPWKWPWWPWRR-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

参考文献
  • [1]. Selsted ME, et al. Indolicidin, a novel bactericidal tridecapeptide amide from neutrophils. J Biol Chem. 1992 Mar 5;267(7):4292-5.

    [2]. Falla TJ, et al. Mode of action of the antimicrobial peptide indolicidin. J Biol Chem. 1996 Aug 9;271(32):19298-303.

Cell Assay
[1]

The time course and dose dependence of indolicidin bactericidal activity are determined using Escherichia coli ML35 and Staphylococcus aureus 502A as test organisms. The assays are performed in 10 mM sodium phosphate buffer, pH 7.4, at 37°C. A 50 μg sample of indolicidin is dissolved in 50 pl of 0.1 M pyridine acetate, pH 6.5, and incubated with 5 μg of chymotrypsin for 15 h at 37°C. Digestion of the sample is confirmed by the disappearance of the indolicidin band on acid-urea PAGE. Following lyophilization, untreated and digested samples are tested for antibacterial activity in a agar diffusion assay against Escherichia coli using concentrations of peptide ranging from 10 to 300 μg/mL[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Selsted ME, et al. Indolicidin, a novel bactericidal tridecapeptide amide from neutrophils. J Biol Chem. 1992 Mar 5;267(7):4292-5.

    [2]. Falla TJ, et al. Mode of action of the antimicrobial peptide indolicidin. J Biol Chem. 1996 Aug 9;271(32):19298-303.