SPACE peptide; 纯度: 98.86%
SPACE peptide 是一种皮肤穿透肽 (SPPs)。SPACE peptide 能增强大分子透明质酸的局部传递。
SPACE peptide Chemical Structure
规格 | 价格 | 是否有货 | 数量 |
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10;mM;*;1 mL in DMSO | ¥7795 | In-stock | |
5 mg | ¥4500 | In-stock | |
10 mg | ¥6500 | In-stock | |
25 mg | ¥12000 | In-stock | |
50 mg | ¥16500 | In-stock | |
100 mg | ; | 询价 | ; |
200 mg | ; | 询价 | ; |
* Please select Quantity before adding items.
SPACE peptide 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
- Peptide Library
生物活性 |
SPACE peptide is a skin penetrating peptide (SPPs). SPACE peptide can enhance topical delivery of a macromolecule, hyaluronic acid[1][2][3]. |
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体外研究 (In Vitro) |
SPACE peptide, when conjugates to cargoes such as small molecules and proteins, is able to facilitate their penetration across the stratum corneum into epidermis and dermis[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
1090.17 |
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Formula |
C40H63N15O17S2 |
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Sequence |
Ala-Cys-Thr-Gly-Ser-Thr-Gln-His-Gln-Cys-Gly (Disulfide bridge: Cys2-Cys10) |
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Sequence Shortening |
ACTGSTQHQCG (Disulfide bridge: Cys2-Cys10) |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:;
DMSO : 100 mg/mL (91.73 mM; Need ultrasonic) H2O : 50 mg/mL (45.86 mM; Need ultrasonic) 配制储备液
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
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参考文献 |
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Cell Assay [2] |
The cytotoxicity of SPACE peptide is assessed using the MTT Cell Proliferation Assay. HEKa cells are seeded in 96-well microplates at a density of 5000 cells/well. Cultures are allowed to grow until they reaches ~80% confluency. Cells are then incubated with 150 μL of 10, 5, 2.5, or 1.25 mg/mL SPACE peptide in media. Media only is used as a negative control, and media without cells is used to subtract background. Cytotoxicity is assessed for 1, 4, and 12 h incubation periods[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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Animal Administration [3] |
Mice: Female BALB/c mice (knockdown of GAPDH protein) are anesthetized using isoflurane inhalation (2-3%), the back skin of animals is shaved, and a cylinder with an exposed skin area of 1.8 cm2 is attached to the back of mice. 200 μL of the SPACE peptide is topically applied in the attached cylinder, and is manually spread over the entire exposure area. Applied test solutions are allowed to incubate with the exposed skin for 6 hours while keeping animals under minimal anesthesia. After 6 hours, the cylinder is carefully removed, and the entire exposure area is covered with sterile gauze and a breathable bandage. After 72 hrs, animals are sacrificed and skin biopsies (5 mm diameter) are collected from treated area of the animal’s skin. Total protein concentration in the skin biopsy tissue is determined[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
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