SPACE peptide

SPACE peptide; 纯度: 98.86%

SPACE peptide 是一种皮肤穿透肽 (SPPs)。SPACE peptide 能增强大分子透明质酸的局部传递。

SPACE peptideamp;;

SPACE peptide Chemical Structure

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥7795 In-stock
5 mg ¥4500 In-stock
10 mg ¥6500 In-stock
25 mg ¥12000 In-stock
50 mg ¥16500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

SPACE peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

SPACE peptide is a skin penetrating peptide (SPPs). SPACE peptide can enhance topical delivery of a macromolecule, hyaluronic acid[1][2][3].

体外研究
(In Vitro)

SPACE peptide, when conjugates to cargoes such as small molecules and proteins, is able to facilitate their penetration across the stratum corneum into epidermis and dermis[2].
SPACE peptide also exhibits increased penetration into various cells including keratinocytes, fibroblasts, and endothelial cells, likely through a macropinocytosis pathway[2].
SPACE peptide enhances Cyclosporine A skin penetration, via a transcellular pathway, enhancing its partitioning into keratin-rich corneocytes through concurrent binding of SPACE with keratin and Cyclosporine A[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1090.17

Formula

C40H63N15O17S2

Sequence

Ala-Cys-Thr-Gly-Ser-Thr-Gln-His-Gln-Cys-Gly (Disulfide bridge: Cys2-Cys10)

Sequence Shortening

ACTGSTQHQCG (Disulfide bridge: Cys2-Cys10)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (91.73 mM; Need ultrasonic)

H2O : 50 mg/mL (45.86 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9173 mL 4.5864 mL 9.1729 mL
5 mM 0.1835 mL 0.9173 mL 1.8346 mL
10 mM 0.0917 mL 0.4586 mL 0.9173 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (2.29 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.29 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (2.29 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.29 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.29 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.29 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Chen M, et al. Topical delivery of hyaluronic acid into skin using SPACE-peptide carriers. J Control Release. 2014 Jan 10;173:67-74.

    [2]. Hsu T, et al. Delivery of siRNA and other macromolecules into skin and cells using a peptide enhancer. Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15816-21.

    [3]. Kumar S, et al. Peptides as skin penetration enhancers: mechanisms of action. J Control Release. 2015 Feb 10;199:168-78.

Cell Assay
[2]

The cytotoxicity of SPACE peptide is assessed using the MTT Cell Proliferation Assay. HEKa cells are seeded in 96-well microplates at a density of 5000 cells/well. Cultures are allowed to grow until they reaches ~80% confluency. Cells are then incubated with 150 μL of 10, 5, 2.5, or 1.25 mg/mL SPACE peptide in media. Media only is used as a negative control, and media without cells is used to subtract background. Cytotoxicity is assessed for 1, 4, and 12 h incubation periods[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice: Female BALB/c mice (knockdown of GAPDH protein) are anesthetized using isoflurane inhalation (2-3%), the back skin of animals is shaved, and a cylinder with an exposed skin area of 1.8 cm2 is attached to the back of mice. 200 μL of the SPACE peptide is topically applied in the attached cylinder, and is manually spread over the entire exposure area. Applied test solutions are allowed to incubate with the exposed skin for 6 hours while keeping animals under minimal anesthesia. After 6 hours, the cylinder is carefully removed, and the entire exposure area is covered with sterile gauze and a breathable bandage. After 72 hrs, animals are sacrificed and skin biopsies (5 mm diameter) are collected from treated area of the animal’s skin. Total protein concentration in the skin biopsy tissue is determined[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Chen M, et al. Topical delivery of hyaluronic acid into skin using SPACE-peptide carriers. J Control Release. 2014 Jan 10;173:67-74.

    [2]. Hsu T, et al. Delivery of siRNA and other macromolecules into skin and cells using a peptide enhancer. Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15816-21.

    [3]. Kumar S, et al. Peptides as skin penetration enhancers: mechanisms of action. J Control Release. 2015 Feb 10;199:168-78.