Kemptide(Synonyms: 肯普肽) | Cytiva思拓凡-Whatman滤纸滤膜滤器

Kemptide;(Synonyms: 肯普肽) 纯度: ge;98.0%

Kemptide是一种合成的七肽，作为cAMP依赖性蛋白激酶 (PKA) 的特异性底物。

Kemptide Chemical Structure

CAS No. : 65189-71-1

规格	价格	是否有货	数量
1 mg	￥500	In-stock
5 mg	￥1100	In-stock
10 mg	￥1900	In-stock
25 mg	￥3900	In-stock
50 mg	;	询价	;
100 mg	;	询价	;

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Kemptide 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus
Peptide Library

生物活性

Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).

体外研究
(In Vitro)

Kemptide is a synthetic construct of a substrate for cAMP-dependent protein kinase (PK). Different types of intact cells catalyze the phosphorylation of Kemptide in the presence of extracellular ATP and cAMP with Km values of 3-4 uM for Kemptide^[1]. Kemptide is a synthetic peptide substrate derived from pyruvate kinase^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

771.91

Formula

C₃₂H₆₁N₁₃O₉

CAS 号

65189-71-1

Sequence

Leu-Arg-Arg-Ala-Ser-Leu-Gly

Sequence Shortening

LRRASLG

中文名称

肯普肽

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-80deg;C	2 years
	-20deg;C	1 year
In solvent	-80deg;C	6 months
	-20deg;C	1 month

溶解性数据

In Vitro:;

H₂O : 50 mg/mL (64.77 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.2955 mL	6.4774 mL	12.9549 mL
5 mM	0.2591 mL	1.2955 mL	2.5910 mL
10 mM	0.1295 mL	0.6477 mL	1.2955 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Kübler D, et al. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55.

[2]. Duncan FE, et al. Transducin-like enhancer of split-6 (TLE6) is a substrate of protein kinase A activity during mouse oocyte maturation. Biol Reprod. 2014 Mar 20;90(3):63.

Kinase Assay ^[2]	PKA activity is measured in pools of 10 oocytes at various stages of maturation using the SignaTECT cAMP-Dependent Protein Kinase (PKA) Assay System. Measurements of basal PKA activity are performed in the absence of exogenous cAMP, whereas measurements of total (cAMP-stimulated) PKA activity are performed in reaction buffer containing 0.025 mM cAMP^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Kübler D, et al. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55. [2]. Duncan FE, et al. Transducin-like enhancer of split-6 (TLE6) is a substrate of protein kinase A activity during mouse oocyte maturation. Biol Reprod. 2014 Mar 20;90(3):63.

Kinase Assay
^[2]

PKA activity is measured in pools of 10 oocytes at various stages of maturation using the SignaTECT cAMP-Dependent Protein Kinase (PKA) Assay System. Measurements of basal PKA activity are performed in the absence of exogenous cAMP, whereas measurements of total (cAMP-stimulated) PKA activity are performed in reaction buffer containing 0.025 mM cAMP^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Kübler D, et al. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55.

[2]. Duncan FE, et al. Transducin-like enhancer of split-6 (TLE6) is a substrate of protein kinase A activity during mouse oocyte maturation. Biol Reprod. 2014 Mar 20;90(3):63.