4-Methylumbelliferyl-β-D-glucuronide hydrate(Synonyms: MUG)

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4-Methylumbelliferyl-β-D-glucuronide hydrate;(Synonyms: MUG) 纯度: 99.98%

4-Methylumbelliferyl-β-D-glucuronide hydrate 是一种荧光底物 (λex=362 nm，λem=445 nm)。

4-Methylumbelliferyl-β-D-glucuronide hydrateamp;;(Synonyms: MUG)

4-Methylumbelliferyl-β-D-glucuronide hydrate Chemical Structure

CAS No. : 881005-91-0

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥790 In-stock
25 mg ￥600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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4-Methylumbelliferyl-beta;-D-glucuronide hydrate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Glycoside Compound Library

生物活性

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).

体外研究
(In Vitro)

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat with λex=362 nm and λem=445 nm. The half-saturation constants, Km, that are calculated for sewage samples are close to the Km values calculated based on experimental plots presented previously for freshwaters[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

370.31

Formula

C16H18O10
CAS 号

881005-91-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 150 mg/mL (405.07 mM; Need ultrasonic)

H2O : 5.2 mg/mL (14.04 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7004 mL 13.5022 mL 27.0044 mL
5 mM 0.5401 mL 2.7004 mL 5.4009 mL
10 mM 0.2700 mL 1.3502 mL 2.7004 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.
Kinase Assay
[1]

GLUase activity of E. coli cells is measured. Three millilitres of 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUGlu) solution (55 mg of hydrated MUGlu and 20 mL of Triton X-100 in 50 mL of sterile water) is added to each flask (final concentration:165 mg/L). The incubation temperature is 44°C. One hundred microlitres of 2 M NaOH solution is added to each 2.9-mL aliquot to obtain a pH>10 before the fluorescence measurement[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.