Dihydrorhodamine 123(Synonyms: DHR 123)

发表于由

Dihydrorhodamine 123;(Synonyms: DHR 123) 纯度: ge;98.0%

Dihydrorhodamine 123 (DHR 123) 是一种荧光探针 (λex=488 nm，λem=525 nm)。

Dihydrorhodamine 123amp;;(Synonyms: DHR 123)

Dihydrorhodamine 123 Chemical Structure

CAS No. : 109244-58-8

规格 价格 是否有货 数量
1 mg ￥500 In-stock
5 mg ; 询价 ;
10 mg ; 询价 ;

* Please select Quantity before adding items.

Dihydrorhodamine 123 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λex=488 nm, λem=525 nm)[1].

体外研究
(In Vitro)

In the presence of 10 μM Dihydrorhodamine 123 (DHR 123) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

346.38

Formula

C21H18N2O3
CAS 号

109244-58-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (288.70 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.8870 mL 14.4350 mL 28.8700 mL
5 mM 0.5774 mL 2.8870 mL 5.7740 mL
10 mM 0.2887 mL 1.4435 mL 2.8870 mL

*

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献

  • [1]. Lydia M. Henderson et al. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur.J.Biochem. 217, 973-980.
Cell Assay
[1]

The HL60 cells are incubated at 6×106 cells/mL in Krebs-Ringer buffer at 37°C containing 10 μM Dihydrorhodamine 123 (DHR). The generation of O2– is initiated by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) and the progress of the generation of rhodamine 123 is monitored in 50-μL aliquots (3×105 cells) diluted tenfold before analysis. The uninduced HL60 cells are loaded with 5 μM carboxy SNARF-1 AM acetate (SNARF-AM) in the Na+ medium for 10 min at 37°C and washed by centrifugation and resuspension to remove unhydrolysed SNARF ester[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Lydia M. Henderson et al. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur.J.Biochem. 217, 973-980.