Rhodamine 123(Synonyms: 罗丹明123 RH-123 R-22420)

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Rhodamine 123;(Synonyms: 罗丹明123; RH-123; R-22420) 纯度: 99.73%

Rhodamine 123 (RH-123; R-22420) 是一种荧光染料（λex=503 nm, λem=527 nm）。

Rhodamine 123amp;;(Synonyms: 罗丹明123; RH-123; R-22420)

Rhodamine 123 Chemical Structure

CAS No. : 62669-70-9

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥550 In-stock
5 mg ￥500 In-stock
10 mg ￥650 In-stock
25 mg ￥900 In-stock
50 mg ￥1500 In-stock
100 mg ￥2500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

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Rhodamine 123 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Cardiovascular Disease Compound Library

生物活性

Rhodamine 123 (RH-123; R-22420) is a fluorescent dye (λex=503 nm, λem=527 nm).

体外研究
(In Vitro)

The intensity of R123 fluorescence has a peak at concentration of 50 μM, and decreases to zero at higher concentrations due to self-quenching[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

380.82

Formula

C21H17ClN2O3
CAS 号

62669-70-9
中文名称

罗丹明123
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (262.59 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6259 mL 13.1296 mL 26.2591 mL
5 mM 0.5252 mL 2.6259 mL 5.2518 mL
10 mM 0.2626 mL 1.3130 mL 2.6259 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285.
Kinase Assay
[1]

Measurements are made at room temperature with continuous stirring of the mitochondrial suspension using spectrophotometer equipped with a magnetic stirrer with fluorescent cation R123 as probe. Excitation and emission wavelengths are 503 nm and 527 nm, respectively. The incubation medium is the respiration buffer. R123 and sodium pyruvate are added to final concentrations of 50 nM and 10 mM, respectively. Isolated mitochondria maintain a steady membrane potential (±5%) throughout the duration of the recording[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285.