TMRE(Synonyms: Tetramethylrhodamine ethyl ester perchlorate)

TMRE;(Synonyms: Tetramethylrhodamine ethyl ester perchlorate) 纯度: 98.24%

TMRE 是一种线粒体特异性染料 (λex=550 nm,λem=575 nm)。

TMREamp;;(Synonyms: Tetramethylrhodamine ethyl ester perchlorate)

TMRE Chemical Structure

CAS No. : 115532-52-0

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥680 In-stock
5 mg ¥600 In-stock
10 mg ¥900 In-stock
25 mg ¥1800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

TMRE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm).

体外研究
(In Vitro)

TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm)[1]. Multidirectional dynamic movement of TMRE is observed in epithelial cells and bidirectional dynamic movement is seen in the superficial cortical fiber cells of live bovine lenses. In the epithelium, the movement of TMRE fluorescence is up to 5 μm/min whereas in the superficial cortex the observed movement is up to 18.5 μm/min. The movement of TMRE fluorescence is abolished with treatment of the uncoupler, carbonyl cyanide m-chlorophenylhydrazone (CCCP)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

514.95

Formula

C26H27ClN2O7

CAS 号

115532-52-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 27.78 mg/mL (53.95 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9419 mL 9.7097 mL 19.4194 mL
5 mM 0.3884 mL 1.9419 mL 3.8839 mL
10 mM 0.1942 mL 0.9710 mL 1.9419 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.04 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.04 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot087361.

    [2]. Bantseev V, et al. Confocal laser scanning microscopy imaging of dynamic TMRE movement in the mitochondria of epithelial and superficial cortical fiber cells of bovine lenses. Mol Vis. 2005 Jul 14;11:518-23.

Cell Assay
[1]

The entire experiment should be performed at room temperature because temperature will directly impact mitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged, incubated, or washed at 4°C or have ice-cold buffers or media added. Treat the cells with a cytotoxic stimulus. Harvest cells and resuspend at 5×105 cells/mL in culture medium containing 150 nM TMRE. Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 μM final concentration) to an aliquot of untreated cells and incubate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Set up a histogram plot to detect TMRE using log scale[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot087361.

    [2]. Bantseev V, et al. Confocal laser scanning microscopy imaging of dynamic TMRE movement in the mitochondria of epithelial and superficial cortical fiber cells of bovine lenses. Mol Vis. 2005 Jul 14;11:518-23.

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