HKOCl-4(Synonyms: BXY2142)

HKOCl-4;(Synonyms: BXY2142)

HKOCl-4 (BXY2142) 是一种以铑为基础的黄色荧光探针,用于检测次氯酸,与荧光素探针相比,具有优异的灵敏度和选择性。HKOCl-4 具有更长的吸收波长和更好的 pH 稳定性。 Ex: 530 nm; Em 557 nm。

HKOCl-4amp;;(Synonyms: BXY2142)

HKOCl-4 Chemical Structure

CAS No. : 2031170-85-9

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1 mg ¥3000 询问价格 货期

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生物活性

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity[1]. HKOCl-4 has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Ex: 530 nm; Em 557 nm.

IC50 Target

Ex: 530 nm; Em 557 nm

体外研究
(In Vitro)

HKOCl-4 (10 μM) shows an increasing florescence intensity with increasing concentration of hypochlorous acid[1].
HKOCl-4 exhibits a ultra-fast response towards 1 equiv of HOCl (reached a plateau within 2 min) , and it shows a >20-fold increase in fluorescence intensity over other analytes (1 equiv of •OH and ONOO− as well as 10 equiv of O2, NO, 1O2, ROO•, TBHP and H2O2)[2].
HKOCl-4 is first tested and it was proven to be non-toxic in living cells even at a concentration up to 50 µM in RAW264.7 macrophages[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

688.59

Formula

C38H35Cl2NO7

CAS 号

2031170-85-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 200 mg/mL (290.45 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4522 mL 7.2612 mL 14.5224 mL
5 mM 0.2904 mL 1.4522 mL 2.9045 mL
10 mM 0.1452 mL 0.7261 mL 1.4522 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 5 mg/mL (7.26 mM); Suspended solution; Need ultrasonic

    此方案可获得 5 mg/mL (7.26 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Dan Yang, et al. Diarylether-based fluorogenic probes for detection of hypochlorous acid or hydroxyl radical. Patent US20160312033.

    [2]. Xiaoyu Bai, et al. HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues.