荧光染料5-FAM SE

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荧光染料Dye Reagents 5-FAM SE; 纯度: ge;95.0%

5-FAM SE 是一种单一异构体，是一种用于标记肽，蛋白质和核苷酸的荧光标记试剂。5-FAM SE 可以与胺反应，并可以产生稳定的胺结合物。

5-FAM SEamp;;

5-FAM SE Chemical Structure

CAS No. : 92557-80-7

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1422 In-stock
10 mg ￥1293 In-stock
50 mg ￥4278 In-stock
100 mg ￥7812 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

5-FAM SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates[1][2].

体外研究
(In Vitro)

The capillary electrophoresis (CE)-laser-induced fluorescent (LIF) method with 5-FAM SE (5-Carboxyfluorescein N-succinimidyl ester) derivatization for the analysis of serotonin can be used to quantify luminally released 5-HT with high sensitivity. CFSE is an excellent dye for the derivatization of 5-HT[1].
5-FAM SE (5-Carboxyfluorescein succinimidyl ester) is chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP) isomers produced after reductive hydrolysis of pheomelanin with hydriodic acid (HI)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

473.39

Formula

C25H15NO9
CAS 号

92557-80-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 25 mg/mL (52.81 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Sheng-da Qi, et al. Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection. Anal Bioanal Chem. 2009 Apr;393(8):2059-66.

    [2]. Xiao-Ling Zhang, et al. Sensitive determination of pheomelanin after 5-carboxyfluorescein succinimidyl ester precapillary derivatization and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jan 1;861(1):136-9.