TRFS-red, a red fluorescence emission off-on probe, is selective for thioredoxin reductase (TrxR). TRFS-red exhibits high response rate and sensitivity. TRFS-red can be used for imaging live cells^[1].

TRFS-red (1 μM; 30-120 min) gives red fluorescence signal in live HeLa cells, and the red fluorescence is predominantly distributed in the cytosol^[1].
TRFS-red (10 μM) has a maximal absorption at ~530 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4)^[1].
TRFS-red displays a reliable fluorescence signal in the pH range of 5.5-8.5^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

682.12

C₂₈H₂₈ClN₃O₁₁S₂

Room temperature in continental US; may vary elsewhere.

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

In Vitro:;

DMSO : 5 mg/mL (7.33 mM; ultrasonic and warming and heat to 60°C)

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: ≥ 0.5 mg/mL (0.73 mM); Clear solution

  此方案可获得 ≥ 0.5 mg/mL (0.73 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 5.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• [1]. Ma H, et, al. A fast response and red emission probe for mammalian thioredoxin reductase. Chem Commun (Camb). 2016 Oct 4;52(81):12060-12063.