荧光染料BODIPY-Cholesterol

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荧光染料Dye Reagents BODIPY-Cholesterol; 纯度: ge;98.0%

BODIPY-cholesterol 是一种带有荧光 BODIPY 基团的亲脂性和细胞通透性胆固醇类似物。BODIPY-cholesterol 可用于监测细胞内固醇摄取和细胞器间固醇通量。(激发/发射波长: 480/508 nm)。

BODIPY-Cholesterol

BODIPY-Cholesterol Chemical Structure

CAS No. : 878557-19-8

规格 价格 是否有货 数量
1 mg ￥4000 In-stock
5 mg ￥9500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

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BODIPY-Cholesterol 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm)[1].

体外研究
(In Vitro)

BODIPY-cholesterol assay
To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM[1].
Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration[1].
Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 µM), mixed thoroughly and incubated for 10 -20 mins at 37 °C[1].
The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

576.61

Formula

C36H51BF2N2O
CAS 号

878557-19-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 1 mg/mL (1.73 mM; Need ultrasonic and warming)

H2O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7343 mL 8.6714 mL 17.3427 mL
5 mM — — —
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Daniel Wüstner, et al.Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28.

    [2]. Maarit Hölttä-Vuori, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organiss. Traffic. 2008 Nov;9(11):1839-49.