HKPerox-1 is a yellow-emitting fluorescent probe with excellent selectivity and sensitivity toward H₂O₂. HKPerox-1 can be used for molecular imaging of endogenous H₂O₂ in living cells^[1].

HKPerox-1 (1-20 μM; 24 h) is nontoxic to RAW264.7 macrophages^[1].
HKPerox-1 (10 μM; 30 min) robustly visualizes endogenous H₂O₂ production in macrophages in a selective manner^[1].
HKPerox-1 should be co-incubated with 100 μM of trichloroacetonitrile (CCl3CN, HY-Y0789) for H₂O₂ detection and molecular imaging.
DMSO should be avoided during H₂O₂ detection, as DMSO could scavenge activated H₂O₂ in the presence of CCl3CN.
DMF is the recommended solvent to prepare probe stock solution.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

714.67

C₄₀H₃₀N₂O₁₁

2240163-05-5

Room temperature in continental US; may vary elsewhere.

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

DMF : 50 mg/mL (69.96 mM; Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: 2.5 mg/mL (3.50 mM); Suspended solution; Need ultrasonic

  此方案可获得 2.5 mg/mL (3.50 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

  Solubility: ≥ 2.5 mg/mL (3.50 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (3.50 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• 3.

  请依序添加每种溶剂：;10% DMF ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: 1.25 mg/mL (1.75 mM); Suspended solution; Need ultrasonic
• 4.

  请依序添加每种溶剂：;10% DMF ;; 90% corn oil

  Solubility: ≥ 1.25 mg/mL (1.75 mM); Clear solution

• [1]. Ye S, et, al. Tandem Payne/Dakin Reaction: A New Strategy for Hydrogen Peroxide Detection and Molecular Imaging. Angew Chem Int Ed Engl. 2018 Aug 6;57(32):10173-10177.