HKSOX-1r (5/6-mixture) is a fluorescent probe which is used for imaging and detection of endogenous superoxide in live cells and in vivo. HKSOX-1r (5/6-mixture) exhibits excellent selectivity and sensitivity towards superoxide anion radical^[1].

HKSOX-1r (5/6-mixture) (2 μM; 30 min) detects mitochondrial respiratory inhibitor-induced O₂^•- formation in a highly sensitive and rapid manner in HCT116, BV-2 and RAW264.7 cells^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

HKSOX-1r (5/6-mixture) (10 μM; 20 min) detects distinct fluorescence distribution in zebrafish embryos are subjected to challenge of PMA or Antimycin A^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

855.54

C₅₈H₃₀F₂₀N₂O₂₈S₄

Room temperature in continental US; may vary elsewhere.

Powder	-20deg;C	3 years
	4deg;C	2 years
In solvent	-80deg;C	6 months
	-20deg;C	1 month

In Vitro:;

DMSO : 20 mg/mL (23.38 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

  Solubility: ≥ 5 mg/mL (5.84 mM); Clear solution

  此方案可获得 ≥ 5 mg/mL (5.84 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Hu JJ, et, al. Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. J Am Chem Soc. 2015 Jun 3;137(21):6837-43.