荧光染料AC-green(Synonyms: VDP-green)

荧光染料Dye Reagents AC-green;(Synonyms: VDP-green)

AC-green (VDP-green) 是一种 β-烯丙基氨基甲酸酯荧光探针,可选择性地对活体系统中的邻位二硫醇蛋白质 (VDPs) 进行成像 (λex/λem=400/475 nm)。AC-green 可以高灵敏度检测降低牛血清白蛋白 (rBSA)。AC-green 毒性低,灵敏度高,适用于检测活细胞和斑马鱼中的 VDP。

AC-green(Synonyms: VDP-green)

AC-green Chemical Structure

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生物活性

AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (λexem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes[1].

体外研究
(In Vitro)

AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts[1].
AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence[1].
AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells[1].
AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols[1].
AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

435.47

Formula

C24H25N3O5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Lanning Zhao, et al. A β-allyl carbamate fluorescent probe for vicinal dithiol proteins. Chem Commun (Camb). 2020 Mar 5;56(19):2857-2860.