荧光染料pyCTZ(Synonyms: Pyridyl CTZ)

荧光染料Dye Reagents pyCTZ;(Synonyms: Pyridyl CTZ)

pyCTZ (Pyridyl CTZ),一种腔肠素 (CTZ) 类似物,是 ATP 非依赖性的 LumiLuc 荧光素酶的吡啶基底物。pyCTZ 在荧光素酶的存在下会产生强烈的蓝色生物发光。 pyCTZ 可用于基于水母发光蛋白的钙感测。

pyCTZ(Synonyms: Pyridyl CTZ)

pyCTZ Chemical Structure

CAS No. : 2125486-34-0

规格 是否有货
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生物活性

pyCTZ (Pyridyl CTZ), a pyridyl Coelenterazine (CTZ) analog, and is an ATP-independent pyridyl substrate of LumiLuc luciferase. pyCTZ generates strong blue bioluminescence in the presence of luciferases. pyCTZ can be used for aequorin-based calcium sensing[1].

体外研究
(In Vitro)

pyCTZ is comparable to native CTZ for bioluminescence intensity when pairs with aequorin[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

392.45

Formula

C25H20N4O

CAS 号

2125486-34-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

溶解性数据
In Vitro:;

Methanol : 41.67 mg/mL (106.18 mM; Need ultrasonic)

DMSO : 33.33 mg/mL (84.93 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5481 mL 12.7405 mL 25.4810 mL
5 mM 0.5096 mL 2.5481 mL 5.0962 mL
10 mM 0.2548 mL 1.2740 mL 2.5481 mL

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请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

参考文献
  • [1]. Yeh HW, et, al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965.