荧光染料Janelia Fluor® 669, SE(Synonyms: JF669, SE; JF669, NHS)

荧光染料Dye Reagents Janelia Fluor® 669, SE;(Synonyms: JF669, SE; JF669, NHS)

Janelia Fluor® 669, SE (JF669, SE) 是一种红色的荧光染料,可与含巯基的卤代标记配体在温和的条件下 (DIEA, DMF) 反应,一步形成 JF669-HaloTag (Ex = 669 nm; Em = 682 nm)。Janelia Fluor® 产品在美国专利下获得许可,来自 Howard Hughes Medical Institute 的 No.9,933,417,10,018,624,10,161,932 和其他专利。

Janelia Fluor® 669, SE(Synonyms: JF669, SE;  JF669, NHS)

Janelia Fluor® 669, SE Chemical Structure

CAS No. : 2127150-20-1

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生物活性

Janelia Fluor® 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669−HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm)[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

体外研究
(In Vitro)

JF669 undergoes rapid conversion to thioether product (<5 min to completion) in organic solvents, where the dye predominantly adopts closed form[1].
JF669 displays comparatively excellent photostability, retaining 97% fluorescence after the same number of cycles[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

693.76

Formula

C34H30F3N3O6SSi

CAS 号

2127150-20-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Jonathan B Grimm, et al. General Synthetic Method for Si-Fluoresceins and Si-Rhodamines. ACS Cent Sci. 2017 Sep 27;3(9):975-985.