荧光染料Tetramethylrhodamine-5-iodoacetamide(Synonyms: 5-TMRIA)

荧光染料Dye Reagents Tetramethylrhodamine-5-iodoacetamide;(Synonyms: 5-TMRIA)

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 是一种选择性巯基反应染料,用于通过半胱氨酸残基对蛋白质进行非特异性标记。Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 可用于共价标记 DNA 片段。

Tetramethylrhodamine-5-iodoacetamide(Synonyms: 5-TMRIA)

Tetramethylrhodamine-5-iodoacetamide Chemical Structure

CAS No. : 114458-99-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments[1].

体外研究
(In Vitro)

Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

569.39

Formula

C26H24IN3O4

CAS 号

114458-99-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Tyagarajan K, et al. Thiol-reactive dyes for fluorescence labeling of proteomic samples.Electrophoresis. 2003 Jul;24(14):2348-58.

发表回复