Biotin-VAD-FMK

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生化分析试剂 Biochemical Assay Reagents
Biotin-VAD-FMK; 纯度: ge;98.0%

Biotin-VAD-FMK是可渗透细胞的，不可逆的，由生物素标记的胱天蛋白酶抑制剂，用于鉴定细胞裂解物中的半胱天冬酶活性。

Biotin-VAD-FMK

Biotin-VAD-FMK Chemical Structure

CAS No. : 1135688-15-1

规格 价格 是否有货 数量
1 mg ￥7000 In-stock
5 mg ￥25000 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Biotin-VAD-FMK 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library

生物活性

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

IC50 Target

Caspase

;

体外研究
(In Vitro)

Biotin-VAD-FMK is a synthetic peptide designed as a methyl ester to facilitate cell permeability.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

To examine whether caspase-2 is activated in the absence of proteolytic cleavage, an in vivo affinity labeling approach is used, using the biotinylated caspase inhibitor biotin-VAD-fmk that detects only active caspases[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

672.81

Formula

C30H49FN6O8S
CAS 号

1135688-15-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 27.3 mg/mL (40.58 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4863 mL 7.4315 mL 14.8630 mL
5 mM 0.2973 mL 1.4863 mL 2.9726 mL
10 mM 0.1486 mL 0.7432 mL 1.4863 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Li J, et al. Nitric oxide suppresses apoptosis via interrupting caspase activation and mitochondrial dysfunction in cultured hepatocytes. J Biol Chem. 1999 Jun 11;274(24):17325-33.

    [2]. Samraj AK, et al. Loss of caspase-9 reveals its essential role for caspase-2 activation and mitochondrial membranedepolarization. Mol Biol Cell. 2007 Jan;18(1):84-93.