Photo-lysine hydrochloride

发表于由

生化分析试剂 Biochemical Assay Reagents
Photo-lysine hydrochloride;

Photo-lysine hydrochloride，一种新的基于赖氨酸光反应性的氨基酸，它捕获结合赖氨酸翻译后修饰的蛋白质。

Photo-lysine hydrochloride

Photo-lysine hydrochloride Chemical Structure

规格 价格 是否有货 数量
5 mg ￥12000 In-stock
10 mg ￥21600 In-stock
20 mg ￥36000 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

体外研究
(In Vitro)

Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

245.11

Formula

C6H14Cl2N4O2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

H2O : ≥ 40 mg/mL (163.19 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.0798 mL 20.3990 mL 40.7980 mL
5 mM 0.8160 mL 4.0798 mL 8.1596 mL
10 mM 0.4080 mL 2.0399 mL 4.0798 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.