Bovine Serum Albumin(Synonyms: 白蛋白; Albumin bovine serum; BSA)

生化分析试剂 Biochemical Assay Reagents
Bovine Serum Albumin;(Synonyms: 白蛋白; Albumin bovine serum; BSA) 纯度: ge;98.0%

Bovine Serum Albumin (BSA) 是由三个同源全 α 结构域组成的 583 个氨基酸的蛋白质。BSA 是一种球状蛋白,可用于多种生物化学实验。

Bovine Serum Albumin(Synonyms: 白蛋白; Albumin bovine serum;  BSA)

Bovine Serum Albumin Chemical Structure

CAS No. : 9048-46-8

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Bovine Serum Albumin 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Bovine Serum Albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. BSA is a globular protein that is used in numerous biochemical applications.

体外研究
(In Vitro)

Bovine serum albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. Bovine serum albumin constitutes ca. 60% of all plasma protein and binds and transports a large number of physiological and non-physiological ligands. Bovine serum albumin contains 17 disulfide bonds and one unpaired cysteine (Cys34), which facilitates dimerization and also influences higher-order association, since the rate of aggregation is slowed down if Cys34 is covalently bound to another compound. MTT assay for fibril cytotoxicity shows Bovine Serum Albumin is beneficial for cell growth irrespective of its aggregated state. Lack of cytotoxicity is confirmed by membrane permeabilization assays. In the subsequent 40 h, an increase in the viability of the treated cells of 300-400% is observed, indicating that the cells incubated with Bovine Serum Albumin achieve a higher viability than the untreated cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号

9048-46-8

中文名称

牛血清白蛋白;牛白蛋白;卵蛋白粉;血清蛋白;血清白蛋白;牛血蛋白质;牛血清蛋白;牛血清蛋白;牛血清清蛋白;白蛋白

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

H2O : ≥ 176.66 mg/mL

* “≥” means soluble, but saturation unknown.

参考文献
  • [1]. Holm NK, et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007 Sep;1774(9):1128-38.

Cell Assay
[1]

Samples are prepared in 96-well microplates and all wells without samples are filled with 200 μL PBS to minimize evaporation. To each well is loaded 100 μL SH-SY5Y growth medium with or without 10.000 SH-SY5Y neuroblastoma cells. The assay is started by adding 25 μL buffer, with or without 5 mg/mL sonicated Bovine Serum Albumin previously incubated for 0, 2 or 96 h at 70°C (giving a final BSA concentration of 1 mg/mL). All sample combinations are loaded in triplets, and for each of the six Bovine Serum Albumin combinations a corresponding triplet is loaded without cells, to be used as blank. One triplet is also loaded without both cells and BSA and one only without Bovine Serum Albumin[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Holm NK, et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007 Sep;1774(9):1128-38.