Geraniin

天然产物 糖类和糖苷 Saccharides and Glycosides

Geraniin; 纯度: 99.58%

Geraniin 是具有抗肿瘤、抗炎、抗高血糖活性的、肿瘤坏死因子α (TNF-α) 的释放抑制剂,其 IC50 值为 43 μM。

Geraniin

Geraniin Chemical Structure

CAS No. : 60976-49-0

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1834 In-stock
5 mg ¥990 In-stock
10 mg ¥1750 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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生物活性

Geraniin is a TNF-α releasing inhibitor with numerous activities including anticancer, anti-inflammatory, and anti-hyperglycemic activities, with an IC50 of 43 μM.

IC50 Target

IC50: 43 μM (TNF-α)[1].

体外研究
(In Vitro)

The IC50 value of TNF-α release inhibition is 43 μM for Geraniin[1]. Geraniin has long been used as a medicinal herb and possesses numerous activities including anticancer, anti-inflammatory, and anti-hyperglycemic activities. Geraniin significantly decreases the viability of OVCAR3 and SKOV3 cells in a concentration-dependent fashion. The IC50 value for Geraniin treatment is 34.5±2.8 μM in OVCAR3 cells and 23.6±1.9 μM in SKOV3 cells. However, Geraniin up to the maximal concentration used (80 μM) has no significant impact on the viability of normal human ovarian surface epithelial cells. Treatment with 10 and 40 μM of Geraniin for 48 h causes a significant increase in apoptosis (16.8±1.2% and 22.6±1.4%, respectively), compared with control OVCAR3 cells (3.9±1.1%). Similar results are observed in SKOV3 cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Treatment with Geraniin prior to application of okadaic acid delays development of tumors compared with control group, reduces the percentage of tumor bearing mice from 80.0% to 40.0%, and reduces the average numbers of tumor per mouse from 3.8 to 1.1 in week 20. It is also showed that oral administration of Geraniin to rats (50 mg/kg/d or 100 mg/kg/d) inhibit the elevation of serum total cholesterol, lipid peroxide, free fatty acid, triglyceride, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase induced by treatment with peroxidized oil[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

952.64

Formula

C41H28O27

CAS 号

60976-49-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (104.97 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0497 mL 5.2486 mL 10.4971 mL
5 mM 0.2099 mL 1.0497 mL 2.0994 mL
10 mM 0.1050 mL 0.5249 mL 1.0497 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (2.62 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.62 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (2.62 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.62 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.62 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.62 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Okabe S, et al. New TNF-alpha releasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki. Biol Pharm Bull. 2001 Oct;24(10):1145-8.

    [2]. Wang X, et al. Geraniin suppresses ovarian cancer growth through inhibition of NF-κB activation and downregulation of Mcl-1 expression. J Biochem Mol Toxicol. 2017 Sep;31(9).

Cell Assay
[2]

Human ovarian cancer cell lines OVCAR3 and SKOV3 are used. Cells are exposed to different concentrations (5, 10, 20, 40, and 80 μM) of Geraniin for 48 h and examined for viability, apoptosis, and gene expression. The concentration range is selected based on previous studies[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Okabe S, et al. New TNF-alpha releasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki. Biol Pharm Bull. 2001 Oct;24(10):1145-8.

    [2]. Wang X, et al. Geraniin suppresses ovarian cancer growth through inhibition of NF-κB activation and downregulation of Mcl-1 expression. J Biochem Mol Toxicol. 2017 Sep;31(9).