天然产物 糖类和糖苷 Saccharides and Glycosides
G-418 disulfate;(Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate) 纯度: 98.26%
G-418 disulfate (Geneticin sulfate) 是一种氨基糖苷类抗生素,抑制真核生物和原核生物的蛋白质合成。G-418 disulfate 是一种常用的真核细胞选择性药剂。
G-418 disulfate Chemical Structure
CAS No. : 108321-42-2
规格 | 价格 | 是否有货 | 数量 |
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Free Sample (0.1-0.5 mg) | ; | Apply now | ; |
10;mM;*;1 mL in Water | ¥500 | In-stock | |
500 mg | ¥400 | In-stock | |
1 g | ¥550 | In-stock | |
5 g | ¥2200 | In-stock | |
10 g | ; | 询价 | ; |
50 g | ; | 询价 | ; |
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G-418 disulfate 相关产品
bull;相关化合物库:
- Natural Product Library Plus
- Bioactive Compound Library Plus
- Anti-Infection Compound Library
- Natural Product Library
- Glycoside Compound Library
- Antibacterial Compound Library
- FDA Approved amp; Pharmacopeial Drug Library
- Antibiotics Library
- Microbial Metabolite Library
生物活性 |
G-418 disulfate (Geneticin sulfate), is an aminoglycoside antibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells[1]. |
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体外研究 (In Vitro) |
G418 sulfate, an aminoglycoside neomycin analogue, interferes with protein synthesis and has been used extensively for selection of mammalian cell lines that possess neomycin resistance (NR). The neomycin resistance (neo) gene is frequently used in eukaryotic vectors as a dominant selectable gene. G418 can be covalently bound to asialoorosomucoid (AsOR) to form a conjugate for hepatocyte-specific targeting and toxicity[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
692.71 |
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Formula |
C20H44N4O18S2 |
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CAS 号 |
108321-42-2 |
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中文名称 |
遗传霉素硫酸盐 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
4deg;C, stored under nitrogen, away from moisture *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (stored under nitrogen, away from moisture) |
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溶解性数据 |
In Vitro:;
H2O : 125 mg/mL (180.45 mM; Need ultrasonic) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
*以上所有助溶剂都可在 MCE 网站选购。
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参考文献 |
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Animal Administration [3] |
To characterize the sensitivity of the trypanosome populations to G418 in vivo, bloodstream forms of T. brucei brucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3 are expanded separately in sublethally irradiated mice. Prior to the first peak of parasitemia, trypanosomes are collected, and aliquots containing 106 trypanosomes are inoculated intraperitoneally into mice. Twenty-four hours following infection, the mice are divided into groups and treated with G418 at a dose of 10, 20, 30, 40, 50, or 80 mg/kg of body weight (bw) by inoculating intraperitoneally 0.2 mL of the drug in sterile water. At 24 and 48 h following the first treatment, G418 is administered to animals in each group at the same dose as before, resulting in three treatments per mouse. Repeated drug treatments are necessary to ensure complete elimination of nontransfected GUTat 3.1 parasites from the mice. Mice are then monitored daily, for 33 days, for the presence of parasites by microscopic examination of wet-blood films. Animals found to be parasitemic are recorded and then removed from the experiment. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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