3′-Methoxypuerarin (3′-MOP) is an isoflavone extracted from radix puerariae that shows neuron protection activity.

3′-Methoxypuerarin increases the number of surviving neurons in the hippocampal CA1 region and markedly reduces the number of apoptotic pyramidal neurons after ischemia/reperfusion injury. 3′-Methoxypuerarin can protect hippocampal neurons against I/R injury by inhibiting apoptosis^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

446.40

C₂₂H₂₂O₁₀

117047-07-1

Room temperature in continental US; may vary elsewhere.

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

In Vitro:;

DMSO : 250 mg/mL (560.04 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: ≥ 2.08 mg/mL (4.66 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (4.66 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.08 mg/mL (4.66 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (4.66 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

  Solubility: ≥ 2.08 mg/mL (4.66 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (4.66 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Liu Y, et al. Protection by 3′-methoxypuerarin of rat hippocampal neurons against ischemia/reperfusion injury. Chin J Physiol. 2010 Apr 30;53(2):136-40.

Rats^[1]

Male Wistar rats, weighing 260-320 g are randomly divided into three groups (n = 8 per group). [1] I/R+3′-MOP group: the rats undergo 8 minutes of ischemia and then 3 days of reperfusion. The 3′-Methoxypuerarin (60 mg/kg i.p.) is administrated at the moment of clamping of the carotid arteries. The rats receive daily i.p. injection of 3′-Methoxypuerarin for 3 days. [2] I/R group: the rats undergo 8 minutes of ischemia and 72 h of reperfusion. The same volume of solvent (35% polyethylene glycol solution) is administrated in the same way as 3′-Methoxypuerarin. [3] Control group: the rats undergo a sham operation. Vertebral and carotid arteries are exposed but not occluded. The rats receive a daily i.p. injection of 35% polyethylene glycol solution for 3 days^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Liu Y, et al. Protection by 3′-methoxypuerarin of rat hippocampal neurons against ischemia/reperfusion injury. Chin J Physiol. 2010 Apr 30;53(2):136-40.