Pulsatilla saponin D(Synonyms: SB365; Hederacolchiside A)

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天然产物 糖类和糖苷 Saccharides and Glycosides

Pulsatilla saponin D;(Synonyms: SB365; Hederacolchiside A) 纯度: 98.47%

Pulsatilla saponin D (SB365) 可从 Pulsatilla koreana Nakai 根部分离得到，具有抗癌活性。

Pulsatilla saponin D(Synonyms: SB365; Hederacolchiside A)

Pulsatilla saponin D Chemical Structure

CAS No. : 68027-15-6

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥3977 In-stock
5 mg ￥1980 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

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Pulsatilla saponin D 相关产品

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生物活性

Pulsatilla saponin D (SB365), isolated from the root of Pulsatilla koreana Nakai, is an anti-tumor agent[1][2][3][4].

体外研究
(In Vitro)

Pulsatilla saponin D (SB365) shows apoptotic effect accompanied by increased evidence of cleaved caspase-3 and poly (ADP ribose) polymerase[1].
Pulsatilla saponin D (SB365) strongly inhibits c-Met expression in gastric cancer cells[1].
Pulsatilla saponin D (SB365) strongly suppresses the growth and proliferation of 5 human pancreatic cancer cell lines (MIAPaCa-2, BXPC-3, PANC-1, AsPC-1 and HPAC) [2].
Pulsatilla saponin D (SB365) shows anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF[3].
Pulsatilla saponin D (0.3 ng/mL to 10 μg/mL) exhibits anti-tumor activity in NSCLC cancer cells[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[4]

Cell Line: A-549, SKMEL-2, MCF-7, and Lewis lung carcinoma (LLC) cells.
Concentration: 0.3 ng/mL to 10 μg/mL.
Incubation Time: 72 h.
Result: Exhibited an ED50 value of 6.3 μg/mL for A-549 cells.

体内研究
(In Vivo)

Pulsatilla saponin D (6.4 mg/kg) exhibits anti-tumor activity in Lewis lung carcinoma model[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 2x2x2 mm3 tumor fragments of a Lewis lung carcinoma (LLC) transplanted s.c. into the auxiliary region of the BDF1 mice[4].
Dosage: 6.4 mg/kg.
Administration: Intraperitoneally on the 1 st to 7 th and 9 th to 14 th day.
Result: Had such a potent antitumor effect (IR, 82 %) on a solid tumor, which is higher than Adriamycin (IR, 64%).

分子量

913.10

Formula

C47H76O17
CAS 号

68027-15-6
中文名称

白头翁皂苷 D
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 39 mg/mL (42.71 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0952 mL 5.4759 mL 10.9517 mL
5 mM 0.2190 mL 1.0952 mL 2.1903 mL
10 mM 0.1095 mL 0.5476 mL 1.0952 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.28 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.28 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.28 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Hong SW, et al. SB365, Pulsatilla saponin D, targets c-Met and exerts antiangiogenic and antitumor activities. Carcinogenesis. 2013 Sep;34(9):2156-69.

    [2]. Son MK, et al. SB365, Pulsatilla saponin D suppresses proliferation and induces apoptosis of pancreatic cancer cells. Oncol Rep. 2013 Aug;30(2):801-8.

    [3]. Son MK, et al. SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway. Food Chem. 2013 Jan 1;136(1):26-33.

    [4]. Yong Kim, et al. Pulsatilla saponin D: the antitumor principle from Pulsatilla koreana. Arch Pharm Res. 2004 Sep;27(9):915-8.