Astragaloside I(Synonyms: Astrasieversianin IV; Cyclosieversioside B)

发表于由

天然产物 糖类和糖苷 Saccharides and Glycosides

Astragaloside I (Synonyms: Astrasieversianin IV; Cyclosieversioside B) 纯度: ≥98.0%

Astragaloside I 是从黄芪中分离出来的一种天然化合物。Astragaloside I 可通过 Wnt/β-catenin 信号通路刺激成骨细胞分化，具有成骨的活性。

Astragaloside I(Synonyms: Astrasieversianin IV; Cyclosieversioside B)

Astragaloside I Chemical Structure

CAS No. : 84680-75-1

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1721 In-stock
5 mg ￥1000 In-stock
10 mg ￥1800 In-stock
50 mg ￥5400 In-stock
100 mg ￥9200 In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Astragaloside I 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Stem Cell Signaling Compound Library
  • Wnt/Hedgehog/Notch Compound Library
  • Natural Product Library
  • Anti-Aging Compound Library
  • Glycoside Compound Library
  • Medicine Food Homology Compound Library
  • Terpenoids Library
  • Cytoskeleton Compound Library
  • Traditional Chinese Medicine Monomer Library
  • Neuroprotective Compound Library
  • Transcription Factor Targeted Library

生物活性

Astragaloside I, one of the main active ingredients in Astragalus membranaceus, has osteogenic properties. Astragaloside I stimulates osteoblast differentiation through the Wnt/β-catenin signaling pathway[1].

体外研究
(In Vitro)

Astragaloside I (10-40 μM) upregulates the express of β-catenin, Runx2, BGP and OPG, RANKL (osteogenesis marker genes) in MC3T3-E1 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1].

Cell Line: MC3T3-E1 cells.
Concentration: 0, 10, 20, 40 μM.
Incubation Time: 5 days.
Result: Stimulated the expression of β-catenin and Runx2.

Cell Cytotoxicity Assay[1].

Cell Line: MC3T3-E1 cells.
Concentration: 0, 10, 20, 40 μM.
Incubation Time: 1, 3 or 6 days (The media was changed every 2 days).
Result: No obvious cytotoxic effect was observed in the MC3T3-E1 cells.

分子量

869.04

Formula

C45H72O16
CAS 号

84680-75-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 50 mg/mL (57.53 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1507 mL 5.7535 mL 11.5070 mL
5 mM 0.2301 mL 1.1507 mL 2.3014 mL
10 mM 0.1151 mL 0.5753 mL 1.1507 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 3.25 mg/mL (3.74 mM); Clear solution

    此方案可获得 ≥ 3.25 mg/mL (3.74 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 3.25 mg/mL (3.74 mM); Clear solution

    此方案可获得 ≥ 3.25 mg/mL (3.74 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Xun Cheng, et al. Astragaloside I Stimulates Osteoblast Differentiation Through the Wnt/β-catenin Signaling Pathway. Phytother Res. 2016 Oct;30(10):1680-1688.