Macelignan(Synonyms: 安五脂素; (+)-Anwulignan; Anwuligan)

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天然产物 天然产物苯丙素类 Phenylpropanoids

Macelignan (Synonyms: 安五脂素; (+)-Anwulignan; Anwuligan) 纯度: ≥98.0%

Macelignan ((+)-Anwulignan; Anwuligan) 是一种从肉豆蔻中分离得到的生物活性木脂素 (lignan)。Macelignan 具有多种药理活性，包括抗炎、抗癌、抗糖尿病和神经保护活性。

Macelignan(Synonyms: 安五脂素; (+)-Anwulignan; Anwuligan)

Macelignan Chemical Structure

CAS No. : 107534-93-0

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1650 In-stock
5 mg ￥1500 In-stock
10 mg ￥2550 In-stock
50 mg   询价  
100 mg   询价  

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生物活性

Macelignan ((+)-Anwulignan; Anwuligan) is an orally active lignan isolated from Myristica fragrans. Macelignan possesses many pharmacological activities, including anti-inflammatory, anti-cancer, anti-diabetes, and neuroprotective activities[1][2][3].

IC50 & Target[1]

COX-2

 

体外研究
(In Vitro)

Macelignan (1-50 μM; 72 hours) does not reduce cell viability alone, however, UVB treatment, even at the lowest dose of 30 mJ/cm2, reduces HaCaT cell viability in a dose-dependent mannner,it reduces approximately 80% of control values at 100 μM in hacat cells[1].
Macelignan (0.1-1 μM; 24 hours) decreases COX-2 expression in a concentration-dependent manner,and at the highest concentration of macelignan (1 μM) , COX-2 expression is inhibited by almost 50% in hacat cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Hacat cells
Concentration: 1 μM; 2.5 μM; 5 μM; 10 μM; 15 μM; 50 μM
Incubation Time: 72 hours
Result: Induced cell death by UVB irradiation at 30 mJ/cm2 from 10 μM.

Western Blot Analysis[1]

Cell Line: Hacat cells
Concentration: 0.1 μM; 0.5 μM;1 μM
Incubation Time: 24 hours
Result: Reduced UVB-induced COX-2 expression in cells.

体内研究
(In Vivo)

Macelignan (oral administration; 15 mg/kg; every day for three weeks) exhibits in vivo anti-diabetic effects. The baseline (day 0) fasting blood glucose levels does not differ between groups; at the end of the experiment, the values of the Macelignan-treated group are significantly lower compared to the diabetic control group in C57BL/KsJ-db/db mice[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/KsJ-db/db mice[2]
Dosage: 15 mg/kg
Administration: Oral administration; 15 mg/kg; every day for three weeks
Result: Significantly reduced the blood glucose levels in mice.

分子量

328.40

Formula

C20H24O4
CAS 号

107534-93-0
中文名称

安五脂素；安五酯素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (304.51 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.0451 mL 15.2253 mL 30.4507 mL
5 mM 0.6090 mL 3.0451 mL 6.0901 mL
10 mM 0.3045 mL 1.5225 mL 3.0451 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (7.61 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (7.61 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (7.61 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Anggakusuma, et al. Effects of macelignan isolated from Myristica fragrans Houtt. on UVB-induced matrix metalloproteinase-9 and cyclooxygenase-2 in HaCaT cells. J Dermatol Sci

    [2]. Jiyoung Yeo, et al. Effects of a multi-herbal extract on type 2 diabetes. Chin Med. 2011 Mar 4;6:10.

    [3]. Chun-Ai Cui, et al. Macelignan attenuates LPS-induced inflammation and reduces LPS-induced spatial learning impairments in rats. Neurosci Lett. 2008 Dec 19;448(1):110-4.