Lupiwighteone(Synonyms: 黄羽扇豆魏特酮; 8-prenylgenistein)

天然产物 黄酮类 Flavonoids

Lupiwighteone (Synonyms: 黄羽扇豆魏特酮; 8-prenylgenistein) 纯度: 98.58%

Lupiwighteone 是一种广泛存在于野生植物中的异黄酮,具有抗氧化、抗菌和抗癌作用。Lupiwighteone 通过抑制 PI3K/Akt/mTOR 通路,诱导人乳腺癌细胞 caspase 依赖性和非依赖性凋亡 (apoptosis)。

Lupiwighteone(Synonyms: 黄羽扇豆魏特酮; 8-prenylgenistein)

Lupiwighteone Chemical Structure

CAS No. : 104691-86-3

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生物活性

Lupiwighteone is an isoflavone present widely in wild-growing plants, with antioxidant, antimicrobial and anticancer effects. Lupiwighteone induces caspase-dependent and -independent apoptosis on human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway[1][2].

IC50 & Target

apoptosis[2]

体外研究
(In Vitro)

Lupiwighteone (2-100 μM; 72 hours) shows cytotoxicity towards various cell lines, especially on DU-145 cells and SGC-7901 cells with IC50s of 23.7 μM and 21 μM, respectively[2].
Lupiwighteone (20-60 μM; 48 hours) induces (cell cycle arrest in DU-145 cells[2].
Lupiwighteone (20-60 μM; 48 hours) induces cells apoptosis[2].
Lupiwighteone (20-60 μM; 48 hours) decreases the cell cycle-related protein expressions in a dose-dependent manner in DU-145 cells[2].
Lupiwighteone (20-60 μM; 48 hours) induces a dose-dependent increase in ROS production[2].
Lupiwighteone up-regulates of cytochrome c and caspase-3, and subsequent cleavage of PARP-1 and down-regulates of the p-Akt/Akt ratio and VEGF expression, suggests the activation of mitochondria-based intrinsic apoptosis in DU-145 cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: DU-145 cells, C4-2 cells, SGC-7901 cells, Hela cells, HUVEC, MCF-7 cells, CNE cells, Vero
Concentration: 2 μM, 5 μM, 25 μM, 50 μM, 100 μM
Incubation Time: 72 hours
Result: Had inhibition on the survival of many cancer cell lines and HUVEC.

Cell Cycle Analysis[2]

Cell Line: DU-145 cells
Concentration: 20 μM, 40 μM, 60 μM
Incubation Time: 48 hours
Result: Induced cell cycle arrest.

Apoptosis Analysis[2]

Cell Line: DU-145 cells
Concentration: 20 μM, 40 μM, 60 μM
Incubation Time: 48 hours
Result: Induced apoptosis.

Western Blot Analysis[2]

Cell Line: DU-145 cells
Concentration: 20 μM, 40 μM, 60 μM
Incubation Time: 48 hours
Result: Decreased CDK1, 2, 4, 6, cyclinD1, and cyclinB1 protein expression in a dose-dependent manner.

分子量

338.35

Formula

C20H18O5

CAS 号

104691-86-3

中文名称

黄羽扇豆魏特酮

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

参考文献
  • [1]. Won YS, et al. Lupiwighteone induces caspase-dependent and -independent apoptosis on human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway. Food Chem Toxicol. 2020 Jan;135:110863.

    [2]. Ren J, et al. Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells. Anticancer Drugs. 2015 Jul;26(6):599-611.