Leachianone A

天然产物 黄酮类 Flavonoids

Leachianone A 

Leachianone A 从槐花中分离,具有抗疟疾,抗炎和细胞毒性作用。 Leachianone A 诱导细胞凋亡 (apoptosis)。

Leachianone A

Leachianone A Chemical Structure

CAS No. : 97938-31-3

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生物活性

Leachianone A, isolated from Radix Sophorae, has anti-malarial, anti-inflammatory, and cytotoxic potent[1]. Leachianone A induces apoptosis involved both extrinsic and intrinsic pathways[2].

体外研究
(In Vitro)

Leachianone A (0-20 μg/ml; 24-72 hours) exhibits a marked inhibition on the survival of HepG2 cells time- and dose-dependently manner, IC50 values are 6.9 μg/ml, 3.4 μg/ml and 2.8 μg/ml in cells with 24-, 48- and 72-hours treatment, respectively[1].
Leachianone A (10-30 μg/ml; 48 hours) indicates that at low concentration of LA (10 μg/ml), a substantial amount of cells is primarily in the early phase of apoptosis, at higher concentrations, induces a shift of the cell population to late apoptotic/ necrotic stage[1].
Leachianone A (10-30 μg/ml; 48 hours) decreases the precursor of caspase-3 in a dose-dependent manner, reduces the protein level of the pro-forms of upstream initiator caspases, caspases-8 and -9, decreases two downstream substrates, namely inhibitor of caspase-activated DNase(ICAD) and poly-ADP-ribose polymerase (PARP) in HepG2 cells[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HepG2 cells
Concentration: 0 μg/ml, 2 μg/ml, 4 μg/ml, 6 μg/ml, 8 μg/ml, 10 μg/ml, 12 μg/ml, 14 μg/ml, 16 μg/ml, 18 μg/ml, 20 μg/ml
Incubation Time: 24-72 hours
Result: Inhibited HepG2 cells survival.

Apoptosis Analysis[1]

Cell Line: HepG2 cells
Concentration: 48 hours
Incubation Time: 10, 20, and 30 μg/ml
Result: Induced the proportion of annexin V-stained cells in both the early and late apoptotic stage.

Western Blot Analysis[1]

Cell Line: HepG2 cells
Concentration: 48 hours
Incubation Time: 10, 20, and 30 μg/ml
Result: Decreased the protein expression of caspase-3, caspases-8 and -9, reduced ICAD and PARP protein expression.

体内研究
(In Vivo)

Leachianone A (intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days) significantly diminishes the tumor volume by 17-54% in LA-treated nude mice, when compared with those solely given the vehicle[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male nude mice with human hepatoma HepG2 cells[1]
Dosage: 20 mg/kg; 30 mg/kg
Administration: Intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days
Result: Suppressed the tumor growth in vivo.

分子量

438.51

Formula

C26H30O6

CAS 号

97938-31-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Jeong GS, et al. Lavandulyl flavanones from Sophora flavescens protect mouse hippocampal cells against glutamate-induced neurotoxicity via the induction of heme oxygenase-1. Biol Pharm Bull. 2008 Oct;31(10):1964-7.

    [2]. Cheung CS, et al. Leachianone A as a potential anti-cancer drug by induction of apoptosis in human hepatoma HepG2 cells. Cancer Lett. 2007 Aug 18;253(2):224-35. Epub 2007 Mar 26.

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