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Products > Optimize Reagents > Optimize – Salts > Sodium malonate

Sodium malonate

Hampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807

CAT NO

HR2-747

NAME

3.4 M Sodium malonate pH 4.0

DESCRIPTION

200 mL

PRICE

$107.00

CAT NO

HR2-749

NAME

3.4 M Sodium malonate pH 5.0

DESCRIPTION

200 mL

PRICE

$107.00

CAT NO

HR2-751

NAME

3.4 M Sodium malonate pH 6.0

DESCRIPTION

200 mL

PRICE

$107.00

CAT NO

HR2-707

NAME

3.4 M Sodium malonate pH 7.0

DESCRIPTION

200 mL

PRICE

$107.00

CAT NO

HR2-807

NAME

3.4 M Sodium malonate pH 8.0

DESCRIPTION

200 mL

PRICE

$107.00

支持材料

Hampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-747 Sodium malonate pH 4.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-749 Sodium malonate pH 5.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-751 Sodium malonate pH 6.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-707 Sodium malonate pH 7.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-807 Sodium malonate pH 8.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 pH/Dilution Table for Sodium Malonate

应用

  • Crystallization grade Sodium malonate for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级丙二酸钠

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

The Sodium malonate offered by Hampton Research is Malonic acid (CAS Number 141-82-2) titrated to pH 4.0-8.0 using Sodium hydroxide as described in the publication “A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.”. In this same publication, Sodium malonate was reported to be more successful than any other salt, resulting in the crystallization of 19 of the 23 macromolecules, almost twice as effective as the next most successful salt, which was a draw between sodium acetate, sodium tartrate, sodium formate, and ammonium sulfate. The high success rate of Sodium malonate in producing crystals was even more impressive when an overall unique success rate with individual macromolecules was considered.

The use of Sodium malonate in place of Ammonium sulfate has been reported in one instance to improve the reproducibility of crystals and improve resistance to physicochemical shocks. Sodium malonate also acted as a good cryoprotectant (Xing and Xu 2003).

One may combine the four different sodium malonate stocks to create a pH versus sodium malonate concentration grid for screening or optimization. Refer to the pH/Dilution Table for Sodium malonate.

Based on the structure of malonic acid titrated with sodium hydroxide, there are no chiral carbons so it is not characterized as having enantiomeric structures, such as D or L.

Hampton Research 提供的丙二酸钠是使用氢氧化钠滴定至 pH 4.0-8.0 的丙二酸(CAS 编号 141-82-2),如出版物“用于大分子结晶的盐的比较。Alexander McPherson。蛋白质科学( 2001), 10:418-422”。在同一份出版物中,据报道丙二酸钠比任何其他盐更成功,导致 23 种大分子中的 19 种结晶,几乎是下一个最成功的盐的两倍,后者是乙酸钠和酒石酸钠之间的抽吸、甲酸钠和硫酸铵。当考虑到单个大分子的整体独特成功率时,丙二酸钠在生产晶体中的高成功率更加令人印象深刻。

据报道,在一个实例中使用丙二酸钠代替硫酸铵可提高晶体的再现性并提高对物理化学冲击的抵抗力。丙二酸钠也是一种很好的冷冻保护剂(Xing and Xu 2003)。

可以将四种不同的丙二酸钠库存组合起来,以创建一个 pH 值与丙二酸钠浓度网格,以进行筛选或优化。请参阅丙二酸钠的 pH/稀释表。

根据用氢氧化钠滴定的丙二酸的结构,没有手性碳,因此不具有对映异构体结构的特征,例如 D 或 L。

Synonyms: Propanedioic acid or Malonic acid
C3H4O4 (before titration with NaOH)
Mr 104.06 (before titration with NaOH)
CAS Number 141-82-2
EC Number 205-503-0
Beilstein Registry Number 1751370
Merck 14,5710
RTECS OO0175000
MDL Number MFCD00002707
Purity > 99.0%
pKa1 2.8
pKa2 5.7

HR2-747 Measured Conductivity Range: 73.3 – 75.3 mS/cm at 25°C
HR2-747 Measured Refractive Index Range: 1.38365 – 1.38425 at 20°C

HR2-749 Measured Conductivity Range: 77.2 – 78.0 mS/cm at 25°C
HR2-749 Measured Refractive Index Range: 1.39264 – 1.39300 at 20°C

HR2-751 Measured Conductivity Range: 73.4 – 75.2 mS/cm at 25°C
HR2-751 Measured Refractive Index Range: 1.39938 – 1.39973 at 20°C

HR2-707 Measured Conductivity Range: 71.0 – 73.7 mS/cm at 25°C
HR2-707 Measured Refractive Index Range: 1.40090 – 1.40098 at 20°C

HR2-807 Measured Conductivity Range: 71.8 – 75.0 mS/cm at 25°C
HR2-807 Measured Refractive Index Range: 1.40106 – 1.40115 at 20°C

参考Sodium malonate

1. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

2. Crystallization of the PX domain of cytokine-independent survival kinase (CISK): improvement of crystal quality for X-ray diffraction with sodium malonate. Yi Xing and Wenqing Xu. Acta Cryst. (2003). D59, 1816-1818.

3. Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A. Petsko. Acta Cryst. (2003). D59, 2356-2358.

4. Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase. Kim et al Acta Cryst. (2007). F63, 579–581.

1. 大分子结晶盐的比较。 亚历山大·麦克弗森。 蛋白质科学(2001),10:418-422。

2. 不依赖细胞因子的存活激酶 (CISK) 的 PX 结构域的结晶:用丙二酸钠改善 X 射线衍射的晶体质量。 艺兴和徐文清。 晶体学报。 (2003 年)。 D59,1816-1818。

3. 丙二酸盐:一种用于盐生大分子晶体的多功能冷冻保护剂和稳定剂。 T. Holyoak、T. D. Fenn、M. A. Wilson、A. G. Moulin、D. Ringe 和 G. A. Petsko。 晶体学报。 (2003 年)。 D59,2356-2358。

4. Est25 的纯化、结晶和初步晶体学分析:一种酮洛芬特异性激素敏感性脂肪酶。 Kim等人Acta Cryst。 (2007 年)。 F63, 579–581。