β-Amyloid (22-35)(Synonyms: Amyloid β-Protein (22-35)) | Cytiva思拓凡-Whatman滤纸滤膜滤器

β-Amyloid (22-35);(Synonyms: Amyloid β-Protein (22-35))

β-Amyloid 22-35 (Amyloid β-Protein 22-35)，β-淀粉样蛋白的 22-35 残基片段，对无血清培养的大鼠海马神经元具有细胞毒性作用。β-Amyloid 22-35 在中性缓冲液中形成聚集体和典型的淀粉样纤维，类似于 β-淀粉样蛋白。

β-Amyloid (22-35)amp;;(Synonyms: Amyloid β-Protein (22-35))

β-Amyloid (22-35) Chemical Structure

CAS No. : 144189-71-9

规格	价格	是否有货
1 mg	￥850	询问价格货期
5 mg	￥3400	询问价格货期
10 mg	￥5800	询问价格货期

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生物活性	β-Amyloid 22-35 (Amyloid β-Protein 22-35), the residues 22-35 fragment ofβ-amyloid protein, has a cytotoxic effect on cultured neurons from the rat hippocampus in serum-free medium. β-Amyloid 22-35 forms aggregates and typical amyloid fibrils resembling those of the β-amyloid protein in neutral buffer solution)^[1].
IC₅₀ Target	Amyloid-β^[1]
体外研究 (In Vitro)	β-Amyloid 22-35 shows significant cytotoxicity on the rat hippocampal neurons at 40 μg/mL, but exhibits no cytotoxic effect on the glial cells^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	1403.62
Formula	C₅₉H₁₀₂N₁₆O₂₁S
CAS 号	144189-71-9
Sequence	Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met
Sequence Shortening	EDVGSNKGAIIGLM
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Takadera T, et al. Toxic effect of a beta-amyloid peptide (beta 22-35) on the hippocampal neuron and its prevention. Neurosci Lett. 1993 Oct 14;161(1):41-4. [2]. Zhou WW, et al. Decreasing oxidative stress and neuroinflammation with a multifunctional peptide rescues memory deficits in mice with Alzheimer disease. Free Radic Biol Med. 2014;74:50-63.

Cell Assay ^[1]	Hippocampal cells are cultured in DMEM supplemented with 10% (v/v) precolostrum newborn calf serum in poly-D-lysine-coated 24-well culture plates at 37°C under a gas mixture of 95% air/5% CO₂. After 6 days of culture, non-neuronal cell division is halted by 24 h exposure to 10^-5 cytosine arabinoside. After washing cell layers with DMEM, cells are cultured with 1 mL of DMEM per well in the absence of serum for 5 days before experiment. Cells are treated for 3 days with β-Amyloid (22-35) or its amidated (C-terminus) derivative by addition of 20 μL of stock solution (2 mg/mL of either in betaine buffer, pH 8.5, consisting of 0.7% betaine and 20 mM NH₄HCO₃) to 1.0 mL medium, unless otherwise specified. Control cells are treated with the betaine buffer alone. Morphological change of cells is checked throughout the course of experiment with a phase-contrast microscope. Dead cells are checked by trypan blue staining. Cell injury is assessed by measuring lactate dehydrogenase (LDH) activity released into medium during 3 days of treatment of neurons with peptides^[1] . MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Takadera T, et al. Toxic effect of a beta-amyloid peptide (beta 22-35) on the hippocampal neuron and its prevention. Neurosci Lett. 1993 Oct 14;161(1):41-4. [2]. Zhou WW, et al. Decreasing oxidative stress and neuroinflammation with a multifunctional peptide rescues memory deficits in mice with Alzheimer disease. Free Radic Biol Med. 2014;74:50-63.

Cell Assay
^[1]

Hippocampal cells are cultured in DMEM supplemented with 10% (v/v) precolostrum newborn calf serum in poly-D-lysine-coated 24-well culture plates at 37°C under a gas mixture of 95% air/5% CO₂. After 6 days of culture, non-neuronal cell division is halted by 24 h exposure to 10^-5 cytosine arabinoside. After washing cell layers with DMEM, cells are cultured with 1 mL of DMEM per well in the absence of serum for 5 days before experiment. Cells are treated for 3 days with β-Amyloid (22-35) or its amidated (C-terminus) derivative by addition of 20 μL of stock solution (2 mg/mL of either in betaine buffer, pH 8.5, consisting of 0.7% betaine and 20 mM NH₄HCO₃) to 1.0 mL medium, unless otherwise specified. Control cells are treated with the betaine buffer alone. Morphological change of cells is checked throughout the course of experiment with a phase-contrast microscope. Dead cells are checked by trypan blue staining. Cell injury is assessed by measuring lactate dehydrogenase (LDH) activity released into medium during 3 days of treatment of neurons with peptides^[1]
.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Takadera T, et al. Toxic effect of a beta-amyloid peptide (beta 22-35) on the hippocampal neuron and its prevention. Neurosci Lett. 1993 Oct 14;161(1):41-4.

[2]. Zhou WW, et al. Decreasing oxidative stress and neuroinflammation with a multifunctional peptide rescues memory deficits in mice with Alzheimer disease. Free Radic Biol Med. 2014;74:50-63.