δ-Sleep Inducing Peptide(Synonyms: Delta-Sleep Inducing Peptide)

δ-Sleep Inducing Peptide;(Synonyms: Delta-Sleep Inducing Peptide)

δ-Sleep Inducing Peptide 是一种神经肽,具有抗氧化和抗焦虑作用。

δ-Sleep Inducing Peptideamp;;(Synonyms: Delta-Sleep Inducing Peptide)

δ-Sleep Inducing Peptide Chemical Structure

CAS No. : 62568-57-4

规格 价格 是否有货
5 mg ¥700 询问价格 货期
10 mg ¥1200 询问价格 货期
25 mg ¥2400 询问价格 货期

* Please select Quantity before adding items.

生物活性

δ-Sleep Inducing Peptide is a neuropeptide, with antioxidant and anxiolytic properties.

体内研究
(In Vivo)

In rabbit infused with δ-Sleep Inducing Peptide (DSIP), the spindle activity is increased, and heart and respiration rate are moderately decreased. δ-Sleep Inducing Peptide (DSIP) may act as a programming modulator at supra-operational level rather than as a transmitter at operational level[1]. δ-Sleep Inducing Peptide (DSIP; 40 μg/kg, i.p.) increases catalase and superoxide dismutase (SOD) activities and malonic dialdehyde (MDA) concentration in the liver homogenate of rats subjected to acute stress, but the opposite effects are observed at 120, and 360 μg/kg[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

848.81

Formula

C35H48N10O15

CAS 号

62568-57-4

Sequence

Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu

Sequence Shortening

WAGGDASGE

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Monnier M, et al. The delta sleep inducing peptide (DSIP). Comparative properties of the original and synthetic nonapeptide. Experientia. 1977 Apr 15;33(4):548-52.

    [2]. Bobyntsev II, et al. Effect of Delta Sleep-Inducing Peptide on Functional State of Hepatocytes in Rats During Restraint Stress. Bull Exp Biol Med. 2016 Feb;160(4):421-4.

Animal Administration
[2]

Rats[2]
Experiments are performed on male Wistar rats (n = 100) weighing 250-280 g. The animals are divided into groups (10 specimens per group). They are maintained under standard vivarium conditions and have free access to water and food (12:12-h light/dark regimen). δ-Sleep Inducing Peptide is dissolved in physiological saline and injected intraperitoneally 60 min before each stress exposure. Control animals receive an equivalent volume of physiological saline (1 mL/kg). The liver is homogenized in ice-cold physiological saline and centrifuged. The content of LPO products and activities of antioxidant enzymes are measured in the supernatant. LPO intensity is evaluated by malonic dialdehyde (MDA) concentration. Activities of superoxide dismutase (SOD) and catalase are measured spectrophotometrically. The total antioxidant activity (TAA) is evaluated by the method based on inhibition of ascorbate-induced and iron-induced oxidation of Tween-80 to MDA. The assay is conducted on a BTS-330 biochemical analyzer[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Monnier M, et al. The delta sleep inducing peptide (DSIP). Comparative properties of the original and synthetic nonapeptide. Experientia. 1977 Apr 15;33(4):548-52.

    [2]. Bobyntsev II, et al. Effect of Delta Sleep-Inducing Peptide on Functional State of Hepatocytes in Rats During Restraint Stress. Bull Exp Biol Med. 2016 Feb;160(4):421-4.