Cerebellin;
Cerebellin是广泛分布于中枢神经系统的神经调节肽。
Cerebellin Chemical Structure
CAS No. : 94071-26-8
规格 | 价格 | 是否有货 | |
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1 mg | ¥2200 | 询问价格 货期 | |
5 mg | ¥6600 | 询问价格 货期 | |
10 mg | ¥9900 | 询问价格 货期 |
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生物活性 |
Cerebellin is a neuromodulatory peptide widely distributed in the central nervous system. |
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体外研究 (In Vitro) |
Cerebellin concentration-dependently (from 1 to 100 nM) increases norepinephrine (but not epinephrine) and cyclic-AMP production by adrenomedullary tissue in vitro[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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体内研究 (In Vivo) |
Cerebellin potently stimulates norepinephrine release by rat adrenal medulla, acting through adenylate-cyclase/PKA-coupled receptors, and enhances adrenocortical steroid secretion in vivo (i.e. when the integrity of adrenal gland is preserved) through an indirect paracrine mechanism involving the release of medullary catecholamines[1]. Cerebellin reduces plasma insulin levels in rats after 1 and 2 h. Cerebellin decreases insulin secretion from isolated rat pancreatic islets at high (11 mM), but not at low (3.33 mM) glucose concentration. Cerebellin inhibits stimulated insulin secretion from clonal rat insulinoma (INS-1) cells, reduces forskolin-induced production of cAMP and intracellular calcium concentration[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
1632.78 |
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Formula |
C69H113N23O23 |
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CAS 号 |
94071-26-8 |
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Sequence |
Ser-Gly-Ser-Ala-Lys-Val-Ala-Phe-Ser-Ala-Ile-Arg-Ser-Thr-Asn-His |
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Sequence Shortening |
SGSAKVAFSAIRSTNH |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis. |
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Solvent Solubility |
In Vitro:;
H2O Peptide Solubility and Storage Guidelines: 1.;;Calculate the length of the peptide. 2.;;Calculate the overall charge of the entire peptide according to the following table:
3.;;Recommended solution:
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参考文献 |
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Kinase Assay [1] |
Some adrenomedullary samples are incubated with 10 μM H89, U-73122 or calphostin-C alone or in the presence of 0.1 μM cerebellin. When cyclic-AMP production is assayed, 100 μM 3-isobutyl-1-methylxanthine is added to prevent cyclic-AMP metabolism by phosphodiesterases. Incubation is carried out for 60 min (aldosterone or corticosterone production), 30 min (catecholamine production) or 10 min (cyclic-AMP production) in a shaking bath at 37°C in an atmosphere of 95% air -5% CO2. At the end of the experiments, the incubation tubes are centrifuged at 4°C, and media are collected and kept frozen at -80°C[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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Animal Administration [2] |
Rats[2] Adult female Wistar rats (180–190 g body weight, 8–12 weeks of age) are injected daily with 0.2 mL of 0.9% NaCl (s.c.) for 14 days. On day 15, animals receive s.c. injections of 0.2 mL of 0.9% NaCl (control groups), while experimental groups are treated with 0.5 or 1.5 nmol/100 g body weight Cerebellin0.5 or 1.5 nmol/100 g body weight Cerebellin. Rats are decapitated 60 or 120 min after injection. Blood is collected into ice-cold tubes containing EDTA (1 mg/mL) and analyzed for the concentration of glucose. Plasma is separated and stored at −80 °C for the determination of the insulin and glucagon concentration[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
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