Malantide TFA

Malantide TFA;

Malantide TFA 是一种合成的十二肽,由被磷酸化酶激酶β亚基上的 PKA 磷酸化的位点衍生而来。Malantide TFA 是 PKA 的高度特异性底物,Km 为 15 μM,并且在各种大鼠组织提取物中显示出 PKI 抑制 > 90%底物磷酸化。Malantide TFA 也是 PKC 的有效底物,Km 为 16 μM。

Malantide TFAamp;;

Malantide TFA Chemical Structure

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Malantide TFA 的其他形式现货产品:

Malantide

生物活性

Malantide TFA is a synthetic dodecapeptide derived from the site phosphorylated by cAMP-dependent protein kinase (PKA) on the β-subunit of phosphorylase kinase. Malantide TFA is a highly specific substrate for PKA with a Km of 15 μM and shows protein inhibitor (PKI) inhibition >90% substrate phosphorylation in various rat tissue extracts[1]. Malantide TFA is also an efficient substrate for PKC with a Km of 16 μM[2].

IC50 Target

Km: 15 μM (PKA)[1], 16 μM (PKC)[2], 233 μM (PKG)[1]

体外研究
(In Vitro)

The Km values of Malantide are 15 μM and 223 μM for PKA and PKG, respectively. The Vmax values are 23.8 units/mg and 6.6 units/mg for for PKA and PKG, respectively[1].
A statistically significant effect of isoprenaline on the activity ratio of guinea-pig heart was only: observed with Malantide[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1747.91

Formula

C74H125F3N22O23

Sequence

Arg-Thr-Lys-Arg-Ser-Gly-Ser-Val-Tyr-Glu-Pro-Leu-Lys-Ile

Sequence Shortening

RTKRSGSVYEPLKI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. K J Murray, et al. Use of a Synthetic Dodecapeptide (Malantide) to Measure the Cyclic AMP-dependent Protein Kinase Activity Ratio in a Variety of Tissues. Biochem J. 1990 May 1;267(3):703-8.

    [2]. Z H Zhao, et al. Characterization of a New Substrate for Protein Kinase C: Assay by Continuous Fluorometric Monitoring and High Performance Liquid Chromatography. Biochem Biophys Res Commun. 1991 May 15;176(3):1454-61.

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