Camstatin TFA

发表于由

Camstatin TFA;

Camstatin TFA 是 PEP-19 智商基序的活性 25-残基片段，结合钙调蛋白并抑制神经元一氧化氮合酶 (NO synthase)。

Camstatin TFAamp;;

Camstatin TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Camstatin TFA, a functionally active 25-residue fragment of PEP-19’s IQ motif, binds calmodulin and inhibits neuronal nitric oxide (NO) synthase[1].

体外研究
(In Vitro)

Camstatin TFA is a synthetic peptide encompassing the minimal IQ motif of PEP-19 that binds apoCaM and inhibits the activities of the CaM-dependent enzymes neuronal nitric oxide synthase (nNOS) and CaM-kinase II[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2874.18

Formula

C124H204F3N39O36
Sequence

Ala-Pro-Glu-Thr-Glu-Arg-Ala-Ala-Val-Ala-Ile-Gln-Ala-Gln-Phe-Arg-Lys-Phe-Gln-Lys-Lys-Lys-Ala-Gly-Ser-NH2
Sequence Shortening

APETERAAVAIQAQFRKFQKKKAGS-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. J Bradley Dickerson, et al. The influence of phosphorylation on the activity and structure of the neuronal IQ motif protein, PEP-19. Brain Res. 2006 May 30;1092(1):16-27.