标签归档：山竹子

Garcinol(Synonyms: 山竹子素)

发表于由

天然产物 黄酮类 Flavonoids

Garcinol (Synonyms: 山竹子素) 纯度: 98.85%

Garcinol 是一种从印度藤黄提取的聚异戊二烯基二苯甲酮，对乙酰胆碱酯酶 (AChE) 和丁酰胆碱酯酶 (BChE) 具有抗胆碱酯酶的特性，IC50 分别为 0.66 µM 和 7.39 µM。Garcinol 还抑制组蛋白乙酰转移酶 (HATs, IC50=7 μM) 和 p300/CPB 相关因子 (PCAF, IC50=5 μM)。Garcinol 具有抗炎和抗癌活性。

Garcinol(Synonyms: 山竹子素)

Garcinol Chemical Structure

CAS No. : 78824-30-3

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥3315 In-stock
1 mg ￥800 In-stock
5 mg ￥2500 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Garcinol 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Epigenetics Compound Library
  • Immunology/Inflammation Compound Library
  • Metabolism/Protease Compound Library
  • Neuronal Signaling Compound Library
  • Natural Product Library
  • Histone Modification Research Compound Library
  • Anti-Cancer Compound Library
  • Human Endogenous Metabolite Compound Library
  • Phenols Library
  • Flavonoids Library
  • Anti-Alzheimer’s Disease Compound Library
  • Neurodegenerative Disease-related Compound Library
  • Food-Sourced Compound Library

生物活性

Garcinol, a polyisoprenylated benzophenone harvested from Garcinia indica, exerts anti-cholinesterase properties towards acetyl cholinesterase (AChE) and butyrylcholinesterase (BChE) with IC50s of 0.66 µM and 7.39 µM, respectively[1]. Garcinol also inhibits histone acetyltransferases (HATs, IC50= 7 μM) and p300/CPB-associated factor (PCAF, IC50 = 5 μM). Garcinol has anti-inflammatory and anti-cancer activity[2].

IC50 & Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Garcinol (10-50 µM; 24-72 hours) can inhibit the proliferation of two HNSCC cell lines tested (CAL27 and UMSCC1) in a time- and dose-dependent manner[3].
Garcinol (10-50 µM; 24-72 hours) induces apoptosis in HNSCC cells[3].
Garcinol (50 µM; 1-6 hours) suppresses phosphorylation and degradation of the constitutive IκBα in a time-dependent manner[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[3]

Cell Line: CAL27 and UMSCC1 cells
Concentration: 10, 25, 50 µM
Incubation Time: 24, 48, and 72 hours
Result: Inhibited the proliferation of two HNSCC cell lines in a time- and dose-dependent manner.

Apoptosis Analysis[3]

Cell Line: CAL27 and UMSCC1 cells
Concentration: 10, 25, 50 µM
Incubation Time: 24, 48, and 72 hours
Result: Induced apoptosis in HNSCC cells.

Western Blot Analysis[3]

Cell Line: CAL27 cells
Concentration: 50 µM
Incubation Time: 1, 2, 4, 6 hours
Result: Suppressed phosphorylation and degradation of the constitutive IκBα in a time-dependent manner.

体内研究
(In Vivo)

Garcinol (i.p.; 1 and 2 mg/kg; five times/week for 4 consecutive weeks) induces significant inhibition of tumor growth[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Five-week-old athymic nu/nu male mice bearing subcutaneous CAL27 tumors[3]
Dosage: 1 and 2 mg/kg
Administration: I.p.; five times/week for 4 consecutive weeks
Result: Induced significant inhibition of tumor growth.

分子量

602.80

Formula

C38H50O6
CAS 号

78824-30-3
中文名称

山竹子素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro: 

DMSO : 50 mg/mL (82.95 mM; Need ultrasonic)

Ethanol : 20 mg/mL (33.18 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6589 mL 8.2946 mL 16.5893 mL
5 mM 0.3318 mL 1.6589 mL 3.3179 mL
10 mM 0.1659 mL 0.8295 mL 1.6589 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (4.15 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.15 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. Lenta BN, et al. Leishmanicidal and cholinesterase inhibiting activities of phenolic compounds from Allanblackia monticola and Symphonia globulifera. Molecules. 2007 Jul 20;12(8):1548-57.

    [2]. Ciochina R, et al. Polycyclic polyprenylated acylphloroglucinols. Chem Rev. 2006 Sep;106(9):3963-86.

    [3]. Li F, et al. Garcinol, a polyisoprenylated benzophenone modulates multiple proinflammatory signalingcascades leading to the suppression of growth and survival of head and neck carcinoma. Cancer Prev Res (Phila). 2013 Aug;6(8):843-54.