标签归档：灵芝

Ganodermanondiol(Synonyms: 灵芝酮二醇)

发表于由

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Ganodermanondiol (Synonyms: 灵芝酮二醇)

Ganodermanondiol 是从灵芝中分离出的一种促黑素生成抑制剂。Ganodermanondiol 对叔丁基过氧化氢诱导的肝毒性具有高效的细胞保护作用。Ganodermanondiol 具有抗 HIV-1 protease 活性，其 IC50 值为 90 μM。Ganodermanondiol 具有很强的抗补体活性，对经典补体途径作用的 IC50 值为 41.7 μM。

Ganodermanondiol(Synonyms: 灵芝酮二醇)

Ganodermanondiol Chemical Structure

CAS No. : 107900-76-5

规格 价格 是否有货
5 mg ￥8500 询问价格 & 货期

* Please select Quantity before adding items.

生物活性

Ganodermanondiol is a melanogenesis inhibitor isolated from the Ganoderma lucidum[1].Ganodermanondiol exhibits potent cytoprotective effects on tert-butyl hydroperoxide-induced hepatotoxicity[2]. Ganodermanondiol shows significant anti-HIV-1 protease activity with an IC50 of 90 μM[3]. Ganodermanondiol exhibits a strong anticomplement activity against the classical pathway of the complement system with an IC50 of 41.7μM[4].

IC50 & Target

IC50: 90μM (HIV-1 protease)[3]
分子量

456.70

Formula

C30H48O3
CAS 号

107900-76-5
中文名称

灵芝酮二醇
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kim JW, et al. Effects of Ganodermanondiol, a New Melanogenesis Inhibitor from the Medicinal Mushroom Ganoderma lucidum. Int J Mol Sci. 2016 Oct 27;17(11).

    [2]. Li B, et al. Protective effect of ganodermanondiol isolated from the Lingzhi mushroom against tert-butyl hydroperoxide-induced hepatotoxicity through Nrf2-mediated antioxidant enzymes. Food Chem Toxicol. 2013 Mar;53:317-24.

    [3]. Min BS, et al. Triterpenes from the spores of Ganoderma lucidum and their inhibitory activity against HIV-1 protease. Chem Pharm Bull (Tokyo). 1998 Oct;46(10):1607-12.

    [4]. Min BS, et al. Anticomplement activity of terpenoids from the spores of Ganoderma lucidum. Planta Med. 2001 Dec;67(9):811-4.

Ganoderic acid D(Synonyms: 灵芝酸 D)

发表于由

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Ganoderic acid D (Synonyms: 灵芝酸 D) 纯度: 99.18%

Ganoderic acid D 是一种高度氧化的四环三萜类化合物 (tetracyclic triterpenoid)，是灵芝 (Ganoderma lucidum) 的主要活性成分。Ganoderic acid D 上调 SIRT3 的蛋白质表达并通过 SIRT3 诱导脱乙酰化的亲环蛋白 D (CypD)。Ganoderic acid D 抑制结肠癌细胞的能量重编程，包括结肠癌细胞中的葡萄糖摄取，乳酸产生，丙酮酸和乙酰辅酶的产生。Ganoderic acid D 诱导 HeLa 人宫颈癌细胞凋亡 (apoptosis)。

Ganoderic acid D(Synonyms: 灵芝酸 D)

Ganoderic acid D Chemical Structure

CAS No. : 108340-60-9

规格 价格 是否有货 数量
1 mg ￥900 In-stock
5 mg ￥2300 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Ganoderic acid D 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Cell Cycle/DNA Damage Compound Library
  • Epigenetics Compound Library
  • Natural Product Library
  • Histone Modification Research Compound Library
  • Anti-Cancer Compound Library
  • Anti-Aging Compound Library
  • Antioxidants Compound Library
  • Lipid Compound Library
  • Oxygen Sensing Compound Library
  • Medicine Food Homology Compound Library
  • Terpenoids Library
  • Glutamine Metabolism Compound Library
  • Traditional Chinese Medicine Monomer Library
  • Anti-Colorectal Cancer Compound Library

生物活性

Ganoderic acid D, a highly oxygenated tetracyclic triterpenoid, is the major active component of Ganoderma lucidum. Ganoderic acid D upregulates the protein expression of SIRT3 and induces the deacetylated cyclophilin D (CypD) by SIRT3. Ganoderic acid D inhibits the energy reprogramming of colon cancer cells including glucose uptake, lactate production, pyruvate and acetyl-coenzyme production in colon cancer cells[1]. Ganoderic acid D induces HeLa human cervical carcinoma apoptosis[2].

IC50 & Target[1]

SIRT3

 

体外研究
(In Vitro)

Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC50 of 17.3 mM[2]. Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner[2].
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest[2].
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells[2].
Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: HeLa human cervical carcinoma cell line (CCL-2)
Concentration: 1, 5, 10, 20, 50 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced the cell survival rate in a dose- and time-dependent manner and had an IC50 value of 17.3 μM for 48 hours treatment.

Cell Cycle Analysis[2]

Cell Line: HeLa human cervical carcinoma cell line (CCL-2)
Concentration: 10, 50 μM
Incubation Time: 24, 48 hours
Result: Induced G2/M phase arrest.
Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM.

Apoptosis Analysis[2]

Cell Line: HeLa human cervical carcinoma cell line (CCL-2)
Concentration: 10, 50 μM
Incubation Time: 48 hours
Result: Induced a morphological change typical of apoptosis in HeLa cells.

Western Blot Analysis[2]

Cell Line: HeLa human cervical carcinoma cell line (CCL-2)
Concentration: 10 μM
Incubation Time: 48 hours
Result: Up-regulated 14-3-3E and PRDX3.

分子量

514.65

Formula

C30H42O7
CAS 号

108340-60-9
中文名称

灵芝酸 D
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (194.31 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9431 mL 9.7153 mL 19.4307 mL
5 mM 0.3886 mL 1.9431 mL 3.8861 mL
10 mM 0.1943 mL 0.9715 mL 1.9431 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (4.86 mM); Clear solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.86 mM) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.86 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.86 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.86 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.86 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. Liu Z, et al. Effect of ganoderic acid D on colon cancer Warburg effect: Role of SIRT3/cyclophilin D. Eur J Pharmacol. 2018 Apr 5;824:72-77.

    [2]. Yue QX, et al. Proteomics characterization of the cytotoxicity mechanism of ganoderic acid D and computer-automated estimation of the possible drug target network. Mol Cell Proteomics. 2008 May;7(5):949-61.

Ganoderic acid B(Synonyms: 灵芝酸B)

发表于由

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Ganoderic acid B (Synonyms: 灵芝酸B) 纯度: 99.31%

Ganoderic acid B 是从灵芝中分离出的一种三萜化合物。Ganoderic acid B 能作为端粒酶抑制剂抑制 Epstein-Barr 病毒 (EBV) 抗原的活化。Ganoderic acid B 是一种 HIV-1 protease 的中等强度抑制剂。

Ganoderic acid B(Synonyms: 灵芝酸B)

Ganoderic acid B Chemical Structure

CAS No. : 81907-61-1

规格 价格 是否有货 数量
1 mg ￥1500 In-stock
5 mg ￥3750 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Ganoderic acid B 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Lipid Compound Library
  • Medicine Food Homology Compound Library
  • Terpenoids Library
  • Traditional Chinese Medicine Monomer Library
  • Microbial Metabolite Library
  • Food-Sourced Compound Library

生物活性

Ganoderic acid B is a triterpene isolated from a mushroom Ganoderma lucidum. Ganoderic acid B inhibits the activation of Epstein-Barr virus (EBV) antigens as telomerase inhibitor. Ganoderic acid B is a moderately active inhibitor against HIV-1 protease[1][2][3].

IC50 & Target

HIV-1 protease[3]
分子量

516.67

Formula

C30H44O7
CAS 号

81907-61-1
中文名称

灵芝酸B
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

DMSO : 50 mg/mL (96.77 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9355 mL 9.6774 mL 19.3547 mL
5 mM 0.3871 mL 1.9355 mL 3.8709 mL
10 mM 0.1935 mL 0.9677 mL 1.9355 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Zhou S, et al. Triterpenes and Soluble Polysaccharide Changes in Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), During Fruiting Growth. Int J Med Mushrooms. 2018;20(9):859-871.

    [2]. Zheng DS, et al. Triterpenoids from Ganoderma lucidum inhibit the activation of EBV antigens as telomerase inhibitors. Exp Ther Med. 2017 Oct;14(4):3273-3278.

    [3]. el-Mekkawy S, et al. Anti-HIV-1 and anti-HIV-1-protease substances from Ganoderma lucidum. Phytochemistry. 1998 Nov;49(6):1651-7.