荧光染料Indo-1 AM(Synonyms: Indo-1 Acetoxymethyl ester)

发表于2024年4月25日由Whatman官网

荧光染料Dye Reagents Indo-1 AM;(Synonyms: Indo-1 Acetoxymethyl ester)

Indo-1 AM 是一种荧光 Ca²⁺ 指示剂 (λ_ex=340 nm，λ_em=405/485 nm)。

Indo-1 AM Chemical Structure

CAS No. : 112926-02-0

规格	价格	是否有货
1 mg	￥6151	询问价格货期

* Please select Quantity before adding items.

生物活性	Indo-1 AM is a fluorescent Ca²⁺ indicator (λ_ex=340 nm, λ_em=405/485 nm).
体外研究 (In Vitro)	Indo-1 AM is a fluorescent Ca²⁺ indicator with λ_ex=340 nm and λ_em=405/485 nm^[1]. In rabbit hearts that are exposed to Indo-1 AM, 72% of the dye is in the cytosol, and the remainder is associated with the mitochondria, microsomes and the nucleus^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	1009.91
Formula	C₄₇H₅₁N₃O₂₂
CAS 号	112926-02-0
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300. [2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.

Cell Assay ^[2]	The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300. [2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.

Cell Assay
^[2]

The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300.

[2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.

Research Diets动物饲料V13002 Formula 模型饲料配方表

发表于2024年4月24日由Whatman官网

上海金畔生物科技有限公司代理Research Diets品牌动物饲料全线产品，欢迎访问官网了解更多产品信息。
Research Diets动物饲料V13002 Formula 模型饲料配方表

V13002

AIN-93 Rodent Vitamin Mix Without Added Vitamin A

适用实验动物模型: 啮齿类 (大鼠/小鼠)
饲料颜色: 未添加染色剂

是否辐照处理/辐照产品号码 :

Research Diets生产饲料大部分都可以送伽马辐照。我们会在这类的产品号结尾标注 “i” 字母以说明其为通过辐照处理的饲料，而饲料经过多轮辐照处理则会标示双字母 “ii” 以区别单轮辐照 “i”。如果您有任何关于辐照的问题，请联系我们的科研技术支持。

无

纯化饲料配方表

营养成分类别	成分	公克
碳水化合物	蔗糖(微粒)	972.73 克
维他命	维生素E醋酸酯, 50%	15.00 克
维他命	Niacin (a.k.a. B3, Nicotinic Acid)	3.00 克
维他命	Vitamin B12, Cyanocobalamin, 0.1%	2.50 克
维他命	生物素, 1%	2.00 克
维他命	D-泛酸半钙盐(维生素 B5)	1.60 克
维他命	维生素D3, 100,000 IU/gm	1.00 克
维他命	吡哆素盐酸盐(维生素B6)	0.70 克
维他命	Riboflavin (A.K.A. B2)	0.60 克
维他命	盐酸硫胺素（维生素B1）	0.60 克
维他命	叶酸	0.20 克
维他命	Phylloquinone (a.k.a. Vitamin K1)	0.08 克
	饲料配方总重（公克）:	1000.00 克

饲料营养素热量组成 Physiological Fuel Values

蛋白质:	0 % 千卡
脂肪:	0 % 千卡
碳水化合物:	100 % 千卡
饲料能量密度:	3.89 千卡/每克

Fluo-3AM(Synonyms: Fluo-3-pentaacetoxymethyl ester)

发表于2024年4月22日由Whatman官网

Fluo-3AM;(Synonyms: Fluo-3-pentaacetoxymethyl ester) 纯度: ge;99.0%

Fluo-3AM(Fluo-3-pentaacetoxymethyl ester)是一种钙指示剂，结合Ca2_addition_能增强其荧光，用于流式细胞仪实验涉及光敏化笼状的螯合剂，第二信使和神经递质以及细胞的药理筛选中Ca2 _addition_信号的空间动态成像。

Fluo-3AMamp;;(Synonyms: Fluo-3-pentaacetoxymethyl ester)

Fluo-3AM Chemical Structure

CAS No. : 121714-22-5

规格	价格	是否有货	数量
500 μg	￥3500	In-stock
1 mg	￥5200	In-stock
5 mg	;	询价	;
10 mg	;	询价	;

* Please select Quantity before adding items.

生物活性

Fluo-3AM(Fluo-3-pentaacetoxymethyl ester) is a calcium indicator that exhibit an increase in fluorescence upon binding Ca2+; used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening.

分子量

1129.85

Formula

C₅₁H₅₀Cl₂N₂O₂₃

CAS 号

121714-22-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据

In Vitro:;

DMSO : 2.5 mg/mL (2.21 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.8851 mL	4.4254 mL	8.8507 mL
5 mM	—	—	—
10 mM	—	—	—

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

Fura-2 AM(Synonyms: Fura-2 Acetoxymethyl ester)

发表于2024年4月15日由Whatman官网

Fura-2 AM;(Synonyms: Fura-2 Acetoxymethyl ester) 纯度: ge;99.0%

Fura-2 AM 是一种高亲和力，细胞内，紫外光激发和 UV 比率荧光 Ca²⁺ 指示剂。

Fura-2 AMamp;;(Synonyms: Fura-2 Acetoxymethyl ester)

Fura-2 AM Chemical Structure

CAS No. : 108964-32-5

规格	价格	是否有货	数量
1 mg	￥4000	In-stock
5 mg	;	询价	;
10 mg	;	询价	;

* Please select Quantity before adding items.

生物活性	Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca²⁺ indicator.
体外研究 (In Vitro)	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid. 1. First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months. 2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min. 3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish. 4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely. 5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish. 6. Mount the coverslip on the imaging chamber. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	1001.85
Formula	C₄₄H₄₇N₃O₂₄
CAS 号	108964-32-5
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	-20deg;C, protect from light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献	[1]. Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23):1067.

Cell Assay	MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23):1067.

BCECF-AM

发表于2024年4月12日由Whatman官网

BCECF-AM;

BCECF-AM 是一种细胞膜穿透的荧光指示剂，可以测量细胞内的 pH 值。

BCECF-AM Chemical Structure

CAS No. : 117464-70-7

规格	价格	是否有货
1 mg	￥3700	询问价格货期

* Please select Quantity before adding items.

生物活性	BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH.
体外研究 (In Vitro)	BCECF-AM is used to measure changes in basal pH_i and NHE activity induced by increasing concentrations of ET-1 (0.1-10 nM) in pulmonary arterial smooth muscle cell (PASMC)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	820.70
Formula	C₈₀H₇₂O₃₈
CAS 号	117464-70-7
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	-20deg;C, protect from light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献	[1]. Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7(9): e46303.

Cell Assay ^[1]	PASMCs are placed in a laminar flow cell chamber perfused with HBSS with pH adjusted to 7.4. pH_i is measured in cells incubated with the membrane permeant (acetoxymethyl ester) form of the pH-sensitive fluorescent dye BCECF-AM for 60 min at 37°C under an atmosphere of 20% O₂-5% CO₂. Cells are then washed with HBSS for 15 min at 37°C to remove extracellular dye and allow complete de-esterification of cytosolic dye. Ratiometric measurement of BCECF fluorescence is performed on a workstation consisting of a Nikon TSE 100 Ellipse inverted microscope with epi-fluorescence attachments. The light beam from a xenon arc lamp is filtered by interference filters at 490 and 440 nm, and focused onto the PASMCS under examination via a 20× fluorescence objective. Light emitted from the cell at 530 nm is returned through the objective and detected by an imaging camera. An electronic shutter is used to minimize photobleaching of dye. Protocols are executed and data collected on-line with InCyte software. pH_i is estimated from in situcalibration after each experiment. Cells are perfused with a solution containing (in mM): 105 KCl, 1 MgCl₂, 1.5 CaCl₂, 10 glucose, 20 HEPES-Tris and 0.01 nigericin to allow pH_i to equilibrate to external pH. A two point calibration is created from fluorescence measured as pH_i is adjusted with KOH from 6.5 to 7.5. Intracellular H⁺ ion concentration ([H⁺]i) is determined from pH_i using the formula: pH_i = −log ([H⁺]i). MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7(9): e46303.

Cell Assay
^[1]

PASMCs are placed in a laminar flow cell chamber perfused with HBSS with pH adjusted to 7.4. pH_i is measured in cells incubated with the membrane permeant (acetoxymethyl ester) form of the pH-sensitive fluorescent dye BCECF-AM for 60 min at 37°C under an atmosphere of 20% O₂-5% CO₂. Cells are then washed with HBSS for 15 min at 37°C to remove extracellular dye and allow complete de-esterification of cytosolic dye. Ratiometric measurement of BCECF fluorescence is performed on a workstation consisting of a Nikon TSE 100 Ellipse inverted microscope with epi-fluorescence attachments. The light beam from a xenon arc lamp is filtered by interference filters at 490 and 440 nm, and focused onto the PASMCS under examination via a 20× fluorescence objective. Light emitted from the cell at 530 nm is returned through the objective and detected by an imaging camera. An electronic shutter is used to minimize photobleaching of dye. Protocols are executed and data collected on-line with InCyte software. pH_i is estimated from in situcalibration after each experiment. Cells are perfused with a solution containing (in mM): 105 KCl, 1 MgCl₂, 1.5 CaCl₂, 10 glucose, 20 HEPES-Tris and 0.01 nigericin to allow pH_i to equilibrate to external pH. A two point calibration is created from fluorescence measured as pH_i is adjusted with KOH from 6.5 to 7.5. Intracellular H⁺ ion concentration ([H⁺]i) is determined from pH_i using the formula: pH_i = −log ([H⁺]i).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7(9): e46303.

Poricoic acid AM

发表于2024年4月12日由Whatman官网

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Poricoic acid AM

Poricic acid AM 是一种具有抗肿瘤活性的三萜类化合物。

Poricoic acid AM Chemical Structure

CAS No. : 151200-92-9

规格		是否有货
5 mg		询价
10 mg		询价
25 mg		询价

* Please select Quantity before adding items.

生物活性	Poricoic acid AM is a triterpenoid with ant-tumor activities^[1].
分子量	512.72
Formula	C₃₂H₄₈O₅
CAS 号	151200-92-9
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Shuang-Shuang Tian, et al. Quality evaluation of Poria based on specific chromatogram and quantitative analysis of multicomponents. Zhongguo Zhong Yao Za Zhi. 2019 Apr;44(7):1371-1380.

BAPTA-AM 品牌：Chemodex

发表于2024年4月4日由Whatman官网

BAPTA-AM

品牌：Chemodex
CAS No.：126150-97-8
储存条件：-20°C
纯度：>95% (HPLC)

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
CDX-B0285-M025	–	25 mg	–	1,410.00	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Ames Test-Positive control AM multi set 致变异性试验用试剂（Ames Test用）

发表于2024年4月4日由Whatman官网

Ames Test-Positive control AM multi set
致变异性试验用试剂（Ames Test用）

产品特性
相关资料
Q&A
参考文献

Ames Test 用阳性对照

Ames Test-Positive control AM multi set

◆特点

调配至可使用浓度的阳性溶液，分别按 0.7 mL 分别分注在血清管中（2 mL 容量），免去最终稀释的时间。溶解后可直接使用。

（附有浓度分析数据及回复试验数据）附有浓度分析数据，可对应 GLP 试验。

◆套装内容

		溶媒	容量	数量
2AA	5 μg/mL	DMSO	0.7 mL	1支
	10 μg/mL	DMSO	0.7 mL	1支
	20 μg/mL	DMSO	0.7 mL	2支
	100 μg/mL	DMSO	0.7 mL	1支
AF-2	0.1 μg/mL	DMSO	0.7 mL	2支
AF-2	1.0 μg/mL	DMSO	0.7 mL	1支
NaN₃	5.0 μg/mL	注射用水	0.7 mL	1支
9AA	800 μg/mL	DMSO	0.7 mL	1支

◆保质期限

　　-80℃以下冻存，可保存19个月

◆相关链接

　　Ames Test概要

产品列表

产品编号	产品名称	产品规格	产品等级	备注
305-51791	Ames Test-Positive control AM multi set	0.7 mL×10	–	–

BAPTA-AM 品牌：Chemodex

发表于2024年4月4日由Whatman官网

BAPTA-AM

品牌：Chemodex
CAS No.：126150-97-8
储存条件：-20°C
纯度：>95% (HPLC)

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
CDX-B0285-M100	–	100 mg	–	5,330.00	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

Ames Test – Climedia AM-N Climedia AM-N培养基/致突变性试验用培养基（Ames Test用）

发表于2024年4月4日由Whatman官网

Ames Test – Climedia AM-N
Climedia AM-N培养基/致突变性试验用培养基（Ames Test用）

产品特性
相关资料
Q&A
参考文献

Climedia AM-N 培养基/致突变性试验用培养基（Ames Test 用）

Ames Test-Climedia AM-N

　　Climedia AM-N 培养基，根据劳动省化学物质调查课编的指导手册做出调整，是利用微生物检测突变性（Ames Test），含有极少量葡萄糖的凝胶型纯培养基。

◆Ames Test 原理及特征

　　致突变性试验与染色体异常试验、遗传因子突变试验有很大区别。

　　Ames Test 是 B.N.Ames 发现的利用微生物检测遗传因子突变性的试验方法，被劳动安全卫生法规定为致癌性的前筛法。

本试验使用鼠伤寒沙门氏菌（Salmonella thphimurium：TA100，TA98，TA1535，TA1537，……）和大肠杆菌（E.coli：WP2uvrA）回复突变试验。

在本培养基上无法合成鼠伤寒沙门氏菌的组氨酸营养缺陷型菌株或大肠杆菌的色氨酸营养缺陷型菌株，但这些试验菌株可通过突变诱发性化学物质引起突变（或恢复），合成组氨酸或色氨酸。菌株数与化学物质的突变诱导性呈比例关系。

Ames Test 比其他致突变性试验简便，化学物质突变诱导性检出更简单、迅速、准确。

另外，本试验不单能检测变异突变性，可用于研究变异突变惰性。

◆组成及调配方法

组成

A	MgSO₄･7H₂O	0.2g
	C₆H₈O₇・H₂O	2g
	Na2HPO4	10g
	NH₄H₂PO₄	1.92g
	NaOH	0.66g
	蒸馏水	200mL
B	葡萄糖	20g
B	蒸馏水	100mL
C	凝胶（伊那食品工业制）	700mL
C	蒸馏水	700mL

　　上述组成的 A、B、C 分别经高压蒸汽灭菌，降温至60℃后混合。

　　分注至放射线灭菌的培养皿中，使其凝固。

◆特点

　　•　产品经过严格的品质管理，任何时候使用都是均一的培养基。

　　•　培养皿、薄膜袋均经放射线灭菌。

◆规格

　　100块（10块装×10袋）

◆使用注意事項

　　•　请按照劳动省化学物质调查课编指导手册，使用本品；

　　•　若本品产生水滴，请在孵化器中充分干燥后使用；

　　•　开封后请尽快使用。

◆储存注意事项

　　•　储存方法：室温（15~25℃），置于阴暗处，避免冷藏。

　　•　气温骤变会产生水滴。

◆相关链接

　　Ames Test概要

产品列表

产品编号	产品名称	产品规格	产品等级	备注
NS69342	Ames Test – Climedia AM-N	100块	–	–

Cytiva思拓凡-Whatman滤纸滤膜滤器

沃特曼官网代理商|Whatman滤纸滤膜中国官网代理商

标签归档：am

荧光染料Indo-1 AM(Synonyms: Indo-1 Acetoxymethyl ester)

Research Diets动物饲料V13002 Formula 模型饲料配方表

V13002

AIN-93 Rodent Vitamin Mix Without Added Vitamin A

纯化饲料配方表

饲料营养素热量组成 Physiological Fuel Values

Fluo-3AM(Synonyms: Fluo-3-pentaacetoxymethyl ester)

Fura-2 AM(Synonyms: Fura-2 Acetoxymethyl ester)

BCECF-AM

Poricoic acid AM

BAPTA-AM 品牌：Chemodex

BAPTA-AM

Ames Test-Positive control AM multi set 致变异性试验用试剂（Ames Test用）

BAPTA-AM 品牌：Chemodex

BAPTA-AM

Ames Test – Climedia AM-N Climedia AM-N培养基/致突变性试验用培养基（Ames Test用）

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