Z-Gly-Gly-Arg-AMC

Z-Gly-Gly-Arg-AMC;

Z-Gly-Gly-Arg-AMC 是凝血素特异性的荧光底物,可用于检测 PRP 和缺乏血小板血浆中凝血酶的生成。

Z-Gly-Gly-Arg-AMCamp;;

Z-Gly-Gly-Arg-AMC Chemical Structure

CAS No. : 66216-78-2

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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Z-Gly-Gly-Arg-AMC 的其他形式现货产品:

Z-Gly-Gly-Arg-AMC acetate

生物活性

Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).

分子量

579.60

Formula

C28H33N7O7

CAS 号

66216-78-2

Sequence

Z-Gly-Gly-Arg-AMC

Sequence Shortening

ZGGR-AMC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Z-DEVD-AMC

Z-DEVD-AMC;

Z-DEVD-AMC 是一种选择性 caspase-3 底物,可通过荧光光谱法测量。 AMC 可用作基于 AMC 的酶底物 (包括基于 AMC 的半胱天冬酶底物) 的荧光参考标准。

Z-DEVD-AMCamp;;

Z-DEVD-AMC Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
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生物活性

Z-DEVD-AMC is a selective caspase-3 substrate that can be measured by fluorescence spectrometry. AMC can be used as a fluorescence reference standard for AMC-based enzyme substrates including AMC-based caspase substrates[1].

分子量

767.74

Formula

C36H41N5O14

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Mauro C X Pinto, et al. Discovery of cytotoxic and pro-apoptotic compounds against leukemia cells: Tert-butyl-4-[(3-nitrophenoxy) methyl]-2,2-dimethyloxazolidine-3-carboxylate. Life Sci. 2011 Nov 21;89(21-22):786-94.

Ac-IETD-AMC

Ac-IETD-AMC;

Ac-IETD-AMC 是一种含有乙酰基 (Ac) 部分的荧光 caspase-8/颗粒酶 B 底物。Ac-IETD-AMC 经常用于测量 caspase-8 活性。

Ac-IETD-AMCamp;;

Ac-IETD-AMC Chemical Structure

CAS No. : 348079-17-4

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Ac-IETD-AMC is a fluorogenic caspase-8/granzyme B substrate containing the acetyl (Ac) moiety. Ac-IETD-AMC is frequently used to measure caspase-8 activity[1].

分子量

675.68

Formula

C31H41N5O12

CAS 号

348079-17-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. V Frost, et al. Exploitation of a non-apoptotic caspase to regulate the abundance of the cdkI p27(KIP1) in transformed lymphoid cells. Oncogene. 2001 May 17;20(22):2737-48.

Boc-Ile-Glu-Gly-Arg-AMC(Synonyms: IEGR-AMC)

Boc-Ile-Glu-Gly-Arg-AMC;(Synonyms: IEGR-AMC)

Boc-Ile-Glu-Gly-Arg-AMC (IEGR-AMC) 是一种激活因子X (FXa) 特异性的荧光肽底物,用于因子 VIII 的测定。

Boc-Ile-Glu-Gly-Arg-AMCamp;;(Synonyms: IEGR-AMC)

Boc-Ile-Glu-Gly-Arg-AMC Chemical Structure

CAS No. : 65147-06-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Boc-Ile-Glu-Gly-Arg-AMC (IEGR-AMC) is an activated factor X (FXa) specific fluorogenic peptide substrate used for Factor VIII determination[1].

分子量

730.81

Formula

C34H50N8O10

CAS 号

65147-06-0

Sequence

Boc-Ile-Glu-Gly-Arg-AMC

Sequence Shortening

Boc-IEGR-AMC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Eich S, et al. Factor VIII determination in patient’s plasma and concentrates: a novel test equally suited for both matrices. Blood Coagul Fibrinolysis. 2003 Jun;14(4):347-53.

Boc-Leu-Gly-Arg-AMC

Boc-Leu-Gly-Arg-AMC; 纯度: 99.64%

Boc-Leu-Gly-Arg-AMC 是一种转化酶的荧光 AMC 底物,可用于酶促测定。

Boc-Leu-Gly-Arg-AMCamp;;

Boc-Leu-Gly-Arg-AMC Chemical Structure

CAS No. : 65147-09-3

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥2150 In-stock
10 mg ¥1950 In-stock
50 mg ¥6600 In-stock
100 mg ¥11500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Boc-Leu-Gly-Arg-AMC 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Endocrinology Compound Library
  • Peptide Library

生物活性

Boc-Leu-Gly-Arg-AMC is a fluorogenic AMC substrate for the convertases. Boc-Leu-Gly-Arg-AMC can be used in enzymatic assays[1][2].

体外研究
(In Vitro)

To demonstrate the presence in the abdominal gland of proteolytic enzymes capable of generating Sodefrin, an enzymatic assay was developed using Boc-Leu-Gly-Arg-AMC as synthetic substrate. A crude extract of the abdominal gland hydrolyzed Boc-Leu-Gly-Arg-AMC to liberate 7-amino-4- methylcoumarin, suggesting that enzymes that generate sodefrin from its precursor molecule are present in the gland. The activity in the extract for cleaving Boc-Leu-Gly-Arg-AMC is optimal at pH 9.0 and 45 ℃[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

601.69

Formula

C29H43N7O7

CAS 号

65147-09-3

Sequence

Boc-Leu-Gly-Arg-AMC

Sequence Shortening

Boc-LGR-AMC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (166.20 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6620 mL 8.3099 mL 16.6199 mL
5 mM 0.3324 mL 1.6620 mL 3.3240 mL
10 mM 0.1662 mL 0.8310 mL 1.6620 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (4.15 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.15 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.15 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.15 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.15 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.15 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Nakada T, et al. Evidence for processing enzymes in the abdominal gland of the newt, Cynops pyrrhogaster, that generate sodefrin from its biosynthetic precursor. Zoolog Sci. 2007 May;24(5):521-4.

    [2]. Yumiko Obayashi, et al. Proteolytic enzymes in coastal surface seawater: Significant activity of endopeptidases and exopeptidases Limnol. Oceanogr., 50(2), 2005, 722-726.

Z-Gly-Gly-Arg-AMC acetate

Z-Gly-Gly-Arg-AMC acetate; 纯度: 98.58%

Z-Gly-Gly-Arg-AMC acetate 是凝血素特异性的荧光底物,可用于检测 PRP 和缺乏血小板血浆中凝血酶的生成。

Z-Gly-Gly-Arg-AMC acetateamp;;

Z-Gly-Gly-Arg-AMC acetate Chemical Structure

CAS No. : 2070009-61-7

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1393 In-stock
5 mg ¥990 In-stock
10 mg ¥1400 In-stock
50 mg ¥4000 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Z-Gly-Gly-Arg-AMC acetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).

分子量

639.66

Formula

C30H37N7O9

CAS 号

2070009-61-7

Sequence

Z-Gly-Gly-Arg-AMC

Sequence Shortening

ZGGR-AMC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 500 mg/mL (781.67 mM; Need ultrasonic)

H2O : 20 mg/mL (31.27 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5633 mL 7.8167 mL 15.6333 mL
5 mM 0.3127 mL 1.5633 mL 3.1267 mL
10 mM 0.1563 mL 0.7817 mL 1.5633 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 4.17 mg/mL (6.52 mM); Clear solution

    此方案可获得 ≥ 4.17 mg/mL (6.52 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 41.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 4.17 mg/mL (6.52 mM); Clear solution

    此方案可获得 ≥ 4.17 mg/mL (6.52 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 41.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 4.17 mg/mL (6.52 mM); Clear solution

    此方案可获得 ≥ 4.17 mg/mL (6.52 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 41.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Tarandovskiy ID, et al. Antiplatelet agents can promote two-peaked thrombin generation in platelet rich plasma: mechanism and possible applications. PLoS One. 2013;8(2):e55688.

    [2]. Machlus KR, et al. Effects of tissue factor, thrombomodulin and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram. Thromb Haemost. 2009 Nov;102(5):936-44.

Ac-DEVD-AMC

Ac-DEVD-AMC; 纯度: 99.45%

Ac-DEVD-AMC 是 Caspase-3 的底物。

Ac-DEVD-AMCamp;;

Ac-DEVD-AMC Chemical Structure

CAS No. : 169332-61-0

规格 价格 是否有货 数量
1 mg ¥500 In-stock
5 mg ¥1500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Ac-DEVD-AMC 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library
  • Peptide Library

生物活性

Ac-DEVD-AMC is the Caspase-3 substrate.

体外研究
(In Vitro)

Ac-DEVD-AMC is the caspase-3 substrate and could be used to monitor intracellular caspase-3 activity[1].The AMC moiety is highest at 2 h (area =5.6±1.8), the AMC peak area for Ac-DEVD-AMC incubated without lung specimen (substrate alone) for 6 h is relatively small[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

675.64

Formula

C30H37N5O13

CAS 号

169332-61-0

Sequence

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin

Sequence Shortening

Ac-DEVD-7-amido-4-Methylcoumarin

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

H2O : 0.67 mg/mL (0.99 mM; Need ultrasonic)

参考文献
  • [1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.

    [2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.

Cell Assay
[2]

Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2 : 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through microcentrifuge filter, separateed on HPLC, and analyzed for the fluorogenic AMC moiety[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.

    [2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.

N-CBZ-Phe-Arg-AMC(Synonyms: Z-FR-AMC)

N-CBZ-Phe-Arg-AMC;(Synonyms: Z-FR-AMC)

N-CBZ-Phe-Arg-AMC (Z-FR-AMC) 是一种组织蛋白酶底物,可用于评估溶酶体组织蛋白酶的活性。

N-CBZ-Phe-Arg-AMCamp;;(Synonyms: Z-FR-AMC)

N-CBZ-Phe-Arg-AMC Chemical Structure

CAS No. : 65147-22-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

N-CBZ-Phe-Arg-AMC (Z-FR-AMC) is a cathepsin substrate used in assessment activity of lysosomal cathepsin enzymes[1][2].

分子量

612.68

Formula

C33H36N6O6

CAS 号

65147-22-0

Sequence

CBZ-Phe-Arg-AMC

Sequence Shortening

CBZ-FR-AMC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Xu P, et al. Ascorbic acid modulation of iron homeostasis and lysosomal function in trabecular meshwork cells. J Ocul Pharmacol Ther. 2014 Mar-Apr;30(2-3):246-53.

    [2]. Porter K,et al. Lysosomal basification and decreased autophagic flux in oxidatively stressed trabecular meshwork cells: implications for glaucoma pathogenesis. Autophagy. 2013 Apr;9(4):581-94.