PKA Inhibitor Fragment (6-22) amide TFA(Synonyms: PKI-(6-22)-amide TFA)

PKA Inhibitor Fragment (6-22) amide TFA;(Synonyms: PKI-(6-22)-amide TFA) 纯度: 96.71%

PKA Inhibitor Fragment (6-22) amide TFA 是 cAMP 依赖性蛋白激酶 A (PKA) 的抑制剂,Ki 值为 2.8 nM。PKA Inhibitor Fragment (6-22) amide TFA 可显着逆转小鼠低水平的吗啡缓解疼痛的感受性。

PKA Inhibitor Fragment (6-22) amide TFAamp;;(Synonyms: PKI-(6-22)-amide TFA)

PKA Inhibitor Fragment (6-22) amide TFA Chemical Structure

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5 mg ¥2100 In-stock
10 mg ¥3500 In-stock
50 mg ; 询价 ;
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PKA Inhibitor Fragment (6-22) amide TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

PKA Inhibitor Fragment (6-22) amide TFA is an inhibitor of cAMP-dependent protein kinase A (PKA), with a Ki of 2.8 nM. PKA Inhibitor Fragment (6-22) amide TFA can significantly reverse low-level morphine antinociceptive tolerance in mice[1][2].

IC50 Target

PKA

2.8 nM (Ki)

分子量

1982.08

Formula

C82H131F3N28O26

Sequence Shortening

TYADFIASGRTGRRNAI-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 50 mg/mL (25.23 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5045 mL 2.5226 mL 5.0452 mL
5 mM 0.1009 mL 0.5045 mL 1.0090 mL
10 mM 0.0505 mL 0.2523 mL 0.5045 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Katz BM, et, al. Synthesis, characterization and inhibitory activities of (4-N3[3,5-3H]Phe10)PKI(6-22)amide and its precursors: photoaffinity labeling peptides for the active site of cyclic AMP-dependent protein kinase. Int J Pept Protein Res. 1989 Jun;33(6):439-45.

    [2]. Dalton GD, et, al. Alterations in brain Protein Kinase A activity and reversal of morphine tolerance by two fragments of native Protein Kinase A inhibitor peptide (PKI). Neuropharmacology. 2005 Apr; 48(5): 648-57.

GLP-1(7-36), amide acetate(Synonyms: Glucagon-like peptide-1 (GLP-1)(7-36), amide acetate Human GLP-1 (7-36), amide acetate)

GLP-1(7-36), amide acetate;(Synonyms: Glucagon-like peptide-1 (GLP-1)(7-36), amide acetate; Human GLP-1 (7-36), amide acetate) 纯度: 98.62%

GLP-1(7-36), amide acetate 是一种主要的肠道激素,在葡萄糖的刺激下,它能促使胰岛 β 细胞分泌胰岛素。

GLP-1(7-36), amide acetateamp;;(Synonyms: Glucagon-like peptide-1 (GLP-1)(7-36), amide acetate; Human GLP-1 (7-36), amide acetate)

GLP-1(7-36), amide acetate Chemical Structure

CAS No. : 1119517-19-9

规格 价格 是否有货 数量
500 μg ¥1200 In-stock
1 mg ¥2000 In-stock
5 mg ¥6000 In-stock
10 mg ¥9500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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GLP-1(7-36), amide acetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

GLP-1(7-36), amide acetate is a major intestinal hormone that stimulates glucose-induced insulin secretion from β cells.

体外研究
(In Vitro)

Cells treated with phorbol 12-myristate 13-acetate for 2 h has significantly higher active GLP-1(7-36) Acetate (Human GLP-1-(7-36)-amide Acetate) concentrations in the media than those in the control. The glucose treatment also increases active GLP-1 secretion from cells in dose-dependent manner. Palmitic, oleic, linoleic or linolenic acid dose-dependently stimulated active GLP-1 secretion from cells. Active GLP-1 secretion is significantly greater with unsaturated fatty acids such as oleic, linoleic and linolenic acids than with palmitic acid. The treatment of NCI-H716 cells with CPE dose-dependently increases active GLP-1 concentrations in the media. A 37% increase is observed in active GLP-1 secretion from these cells at a concentration of 0.1 % CPE[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Gastric administration of glucose increases active GLP-1(7-36) amide levels in the portal blood after 10 min, followed by a marked decrease at 30 min. The gastric administration of TO also increases active GLP-1 levels after 10 min, and followed by a decrease to basal levels at 60 min. Individually, glucose and TO increase the secretion of GLP-1 in a dose-dependent manner. Furthermore, the co-administration of glucose and TO additively increase peak GLP-1 levels. CPE-administered mice have higher active GLP-1 levels in the portal blood at 10 and 30 min than those in the control mice. When glucose is administered with CPE, active GLP-1 and insulin levels in the portal blood are slightly higher in CPE-administered mice than in the control mice. High-fat diet-fed C57BL/6J mice develop hyperglycaemia and impair glucose tolerance[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

3357.73

Formula

C149H226N40O45.xC2H4O2

CAS 号

1119517-19-9

Sequence

His-Ala-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu-Val-Lys-Gly-Arg-NH2

Sequence Shortening

HAEGTFTSDVSSYLEGQAAKEFIAWLVKGRNH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

H2O : 100 mg/mL (29.78 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2978 mL 1.4891 mL 2.9782 mL
5 mM 0.0596 mL 0.2978 mL 0.5956 mL
10 mM 0.0298 mL 0.1489 mL 0.2978 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Fujii Y et al. Ingestion of coffee polyphenols increases postprandial release of the active glucagon-like peptide-1(GLP-1(7-36)) amide in C57BL/6J mice. J Nutr Sci. 2015 Mar 3

GIP (1-30) amide,human acetate

GIP (1-30) amide,human acetate; 纯度: 98.26%

GIP (1-30) amide,human acetate 是葡萄糖依赖性促胰岛素多肽 (GIP) 片段。葡萄糖依赖性促胰岛素多肽是一种肠降血糖素激素,可刺激胰岛素分泌并减少餐后血糖波动。GIP (1-30) amide,human acetate 在 10-9-10-6 M 范围内剂量依赖性地促进胰岛素分泌。

GIP (1-30) amide,human acetateamp;;

GIP (1-30) amide,human acetate Chemical Structure

规格 价格 是否有货 数量
1 mg ¥1500 In-stock
5 mg ¥4500 In-stock
10 mg ¥7500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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GIP (1-30) amide,human acetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

GIP (1-30) amide,human acetate is a glucose-dependent insulinotropic polypeptide (GIP) fragment. GIP is an incretin hormone that stimulates insulin secretion and reduces postprandial glycaemic excursions. GIP (1-30) amide,human acetate dose-dependently promotes insulin secretion over the range 10-9-10-6 M[1].

体外研究
(In Vitro)

The glucose-dependent action of Glucose-dependent insulinotropic polypeptide (GIP) on pancreatic β-cells has attracted attention towards its exploitation as a potential drug for type 2 diabetes. In a 50% aqueous trifluoroethanol solvent, GIP(1-30) amide has an α-helical structural region from F6 to A28. The structures calculated for GIP(1-30) amide remain within one family of conformations and the level of agreement between the structures demonstrated the ordered arrangement[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

3591.99

Formula

C164H244N40O49S

Sequence

Tyr-Ala-Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His-Gln-Gln-NH2

Sequence Shortening

YAEGTFISDYSIAMDKIHQQDFVNWLLAQK-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

DMSO : 50 mg/mL (13.92 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2784 mL 1.3920 mL 2.7840 mL
5 mM 0.0557 mL 0.2784 mL 0.5568 mL
10 mM 0.0278 mL 0.1392 mL 0.2784 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Alaña I, et al. NMR structure of the glucose-dependent insulinotropic polypeptide fragment, GIP(1-30)amide. Biochem Biophys Res Commun. 2004 Dec 3;325(1):281-6.

[Met5]-Enkephalin, amide TFA(Synonyms: 5-Methionine-enkephalin amide TFA)

[Met5]-Enkephalin, amide TFA;(Synonyms: 5-Methionine-enkephalin amide TFA) 纯度: 99.71%

[Met5]-Enkephalin, amide TFA 是一种 δζ 阿片受体 (opioid receptor) 激动剂。

[Met5]-Enkephalin, amide TFAamp;;(Synonyms: 5-Methionine-enkephalin amide TFA)

[Met5]-Enkephalin, amide TFA Chemical Structure

规格 价格 是否有货 数量
10 mg ¥600 In-stock
25 mg ¥1200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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[Met5]-Enkephalin, amide TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

[Met5]-Enkephalin, amide TFA is an agonist for δ opioid receptors as well as putative ζ (zeta) opioid receptors.

IC50 Target

δ and ζ opioid receptor[1]

体外研究
(In Vitro)

[Met5]-Enkephalin at 0.1 nM, 10 nM, and 1 μM significantly reduces the total number of glial cells in culture[1]. [Met5]-Enkephalin, amide acts via δ-opioid receptor to inhibit pelvic nerve-evoked contractions of cat distal colon. [Met5]-enkephalin causes concentration-dependent, reversible inhibition of pelvic nerve-evoked contractions, with an IC50 value of 2.2 nM. [Met5]enkephalin at a concentration (3 nM) which produces a large inhibition of neurogenic contractions, has no effect on contractions to exogenous acetylcholine[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

686.70

Formula

C29H37F3N6O8S

Sequence

Tyr-Gly-Gly-Phe-Met-NH2

Sequence Shortening

YGGFM-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 100 mg/mL (145.62 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4562 mL 7.2812 mL 14.5624 mL
5 mM 0.2912 mL 1.4562 mL 2.9125 mL
10 mM 0.1456 mL 0.7281 mL 1.4562 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Stiene-Martin A, et al. Glial growth is regulated by agonists selective for multiple opioid receptor types in vitro. J Neurosci Res. 1991 Aug;29(4):538-48.

    [2]. Kennedy C, et al. [Met5]enkephalin acts via delta-opioid receptors to inhibit pelvic nerve-evoked contractions of cat distal colon. Br J Pharmacol. 1987 Oct;92(2):291-8.

GLP-1(7-36), amide TFA(Synonyms: Glucagon-like peptide-1 (GLP-1)(7-36), amide TFA Human GLP-1 (7-36), amide TFA)

GLP-1(7-36), amide TFA;(Synonyms: Glucagon-like peptide-1 (GLP-1)(7-36), amide TFA; Human GLP-1 (7-36), amide TFA) 纯度: 99.20%

GLP-1(7-36), amide TFA 是一种主要的肠道激素,在葡萄糖的刺激下,它能促使胰岛 β 细胞分泌胰岛素。

GLP-1(7-36), amide TFAamp;;(Synonyms: Glucagon-like peptide-1 (GLP-1)(7-36), amide TFA; Human GLP-1 (7-36), amide TFA)

GLP-1(7-36), amide TFA Chemical Structure

规格 价格 是否有货 数量
500 μg ¥1200 In-stock
1 mg ¥2000 In-stock
5 mg ¥6000 In-stock
10 mg ¥9500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

GLP-1(7-36), amide TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

GLP-1(7-36), amide TFA is a major intestinal hormone that stimulates glucose-induced insulin secretion from β cells[1].

体外研究
(In Vitro)

Cells treated with phorbol 12-myristate 13-acetate for 2 h has significantly higher active GLP-1(7-36) Acetate (Human GLP-1-(7-36)-amide Acetate) concentrations in the media than those in the control. The glucose treatment also increases active GLP-1 secretion from cells in dose-dependent manner. Palmitic, oleic, linoleic or linolenic acid dose-dependently stimulated active GLP-1 secretion from cells. Active GLP-1 secretion is significantly greater with unsaturated fatty acids such as oleic, linoleic and linolenic acids than with palmitic acid. The treatment of NCI-H716 cells with CPE dose-dependently increases active GLP-1 concentrations in the media. A 37% increase is observed in active GLP-1 secretion from these cells at a concentration of 0.1 % CPE[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Gastric administration of glucose increases active GLP-1(7-36) amide levels in the portal blood after 10 min, followed by a marked decrease at 30 min. The gastric administration of TO also increases active GLP-1 levels after 10 min, and followed by a decrease to basal levels at 60 min. Individually, glucose and TO increase the secretion of GLP-1 in a dose-dependent manner. Furthermore, the co-administration of glucose and TO additively increase peak GLP-1 levels. CPE-administered mice have higher active GLP-1 levels in the portal blood at 10 and 30 min than those in the control mice. When glucose is administered with CPE, active GLP-1 and insulin levels in the portal blood are slightly higher in CPE-administered mice than in the control mice. High-fat diet-fed C57BL/6J mice develop hyperglycaemia and impair glucose tolerance[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

3411.65

Formula

C151H227F3N40O47

Sequence Shortening

HAEGTFTSDVSSYLEGQAAKEFIAWLVKGRNH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Fujii Y et al. Ingestion of coffee polyphenols increases postprandial release of the active glucagon-like peptide-1(GLP-1(7-36)) amide in C57BL/6J mice. J Nutr Sci. 2015 Mar 3;4:e9.

ABL-001-Amide-PEG3-acid

ABL-001-Amide-PEG3-acid;

ABL-001-Amide-PEG3-acid 是ABL-001 的类似物,常被用作标记的化学或荧光探针。

ABL-001-Amide-PEG3-acidamp;;

ABL-001-Amide-PEG3-acid Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

ABL-001-Amide-PEG3-acid, an analogue of ABL-001, is usually used as a labeled chemical or fluorescent probe.

分子量

683.06

Formula

C29H33ClF2N6O9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

WRW4, Amide 品牌:Anygen


WRW4, Amide

品牌:Anygen
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

303-92131

0.5 mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

Galanin(1-13)-P-P-(A-L)2-A-Amide, M40 品牌:Anygen


Galanin(1-13)-P-P-(A-L)2-A-Amide, M40

品牌:Anygen
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

307-36591

0.5 mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

FMRF-Amide 品牌:Peptide


FMRF-Amide

品牌:Peptide
CAS No.:64190-70-1
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

337-41423

25mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

GRF (1-29) amide (rat)(Synonyms: rGHRH(1-29)NH2)

GRF (1-29) amide (rat);(Synonyms: rGHRH(1-29)NH2) 纯度: 98.22%

GRF (1-29) amide (rat) 是能刺激生长激素 (GH) 分泌的合成肽。

GRF (1-29) amide (rat)amp;;(Synonyms: rGHRH(1-29)NH2)

GRF (1-29) amide (rat) Chemical Structure

CAS No. : 91826-20-9

规格 价格 是否有货 数量
1 mg ¥1100 In-stock
5 mg ¥2200 In-stock
10 mg ¥3500 In-stock
25 mg ¥7500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

GRF (1-29) amide (rat) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

GRF (1-29) amide (rat) is a synthetic peptide which can stimulate the growth hormone (GH) secretion.

体内研究
(In Vivo)

Time course studies of GRF (1-29) amide (rat) disappearance show apparent half-lives of 18±4 min and 13±3 min in serum and liver homogenate, respectively. This is accompanied by the appearance of degradation products that are all less hydrophobic than the native peptide. In the serum, two major metabolites are detected and isolated by preparative HPLC[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

3473.08

Formula

C155H251N49O40S

CAS 号

91826-20-9

Sequence

His-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu-Ile-Met-Asn-Arg-NH2

Sequence Shortening

HADAIFTSSYRRILGQLYARKLLHEIMNR-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 16.67 mg/mL (4.80 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2879 mL 1.4396 mL 2.8793 mL
5 mM
10 mM

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Boulanger L, et al. Catabolism of rat growth hormone-releasing factor(1-29) amide in rat serum and liver. Peptides. 1992 Jul-Aug;13(4):681-9.

Cell Assay
[1]

To isolate GRF metabolites in the liver, rGRF(1-29)NH2 (10 mg) is first preincubated in 232 mL of Krebs’ buffer (5 min, 37°C) to help GRF solubilization and then incubated with a liver homogenate in a shaking bath at 37°C. The homogenate is prepared as in the degradation assays with 580 mg of liver (10 mg/mL). The reaction is stopped after 30 min by adding 174 mL of cold 50 mM phosphate solution (pH 0.8) and centrifugation (48,000×g, 20 min, 4°C). The supernatant is filtered twice and its pH is adjusted (3.0) with 6 N NaOH before chromatography. The GRF metabolites and residual rGRF(1-29)NH2 are isolated[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Boulanger L, et al. Catabolism of rat growth hormone-releasing factor(1-29) amide in rat serum and liver. Peptides. 1992 Jul-Aug;13(4):681-9.