标签归档：analog

Dermorphin Analog

发表于由

Dermorphin Analog;

Dermorphin Analog 是 Dermorphin 的类似物。Dermorphin 为两栖类皮肤中发现的天然七肽，为 μ-阿片受体 (μ-opioid receptor) 激动剂。

Dermorphin Analogamp;;

Dermorphin Analog Chemical Structure

规格 价格 是否有货
1 mg ￥900 询问价格 货期
5 mg ￥1800 询问价格 货期
10 mg ￥2800 询问价格 货期

* Please select Quantity before adding items.

生物活性

Dermorphin Analog is an analog of Dermorphin. Dermorphin is a natural heptapeptide μ-opioid receptor agonist found in amphibian skin.

分子量

901.43

Formula

C44H59N11O10
Sequence

Tyr-{d-Arg}-Phe-{SAR}-Tyr-Pro-Ser-NH2
Sequence Shortening

Y-{d-Arg}-F-{SAR}-YPS-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Cardiotoxin Analog (CTX) IV (6-12)

发表于由

Cardiotoxin Analog (CTX) IV (6-12); 纯度: 98.18%

Cardiotoxin Analog (CTX) IV (6-12) 是心脏毒素类似物IV。Cardiotoxin analogues IV是从台湾眼镜蛇的毒液中分离出来，是一种独特的蛇毒心脏毒素。

Cardiotoxin Analog (CTX) IV (6-12)amp;;

Cardiotoxin Analog (CTX) IV (6-12) Chemical Structure

CAS No. : 115722-23-1

规格 价格 是否有货 数量
1 mg ￥700 In-stock
5 mg ￥2000 In-stock
10 mg ￥3600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Cardiotoxin Analog (CTX) IV (6-12) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Cardiotoxin Analog (CTX) IV (6-12) is a part peptide of Cardiotoxin Analog (CTX) IV. Cardiotoxin analogues IV isolated from the venom of Taiwan Cobra. CTX IV is an unique snake venom cardiotoxin[1].

分子量

899.13

Formula

C48H70N10O7
CAS 号

115722-23-1
Sequence

Leu-Ile-Pro-Pro-Phe-Trp-Lys-NH2
Sequence Shortening

LIPPFWK-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 100 mg/mL (111.22 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1122 mL 5.5609 mL 11.1219 mL
5 mM 0.2224 mL 1.1122 mL 2.2244 mL
10 mM 0.1112 mL 0.5561 mL 1.1122 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Kaneda N, et al. The amino acid sequence of cardiotoxin-analogue IV from the venom of Naja naja atra. FEBS Lett. 1976 Nov;70(1):217-22.

荧光染料Hoechst 33342 analog

发表于由

荧光染料Dye Reagents Hoechst 33342 analog; 纯度: 98.04%

Hoechst 33342 analog 是 Hoechst 33342 的一种类似物, Hoechst 33342 结合到 DNA 小沟，用于细胞 DNA 荧光染色。

Hoechst 33342 analogamp;;

Hoechst 33342 analog Chemical Structure

CAS No. : 178481-68-0

规格 价格 是否有货 数量
5 mg ￥5000 In-stock
10 mg ￥8500 In-stock
25 mg ￥18000 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33342 analog 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33342 analog is an anglog of Hoechst 33342, which is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

分子量

590.59

Formula

C32H37Cl2N7
CAS 号

178481-68-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (169.32 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6932 mL 8.4661 mL 16.9322 mL
5 mM 0.3386 mL 1.6932 mL 3.3864 mL
10 mM 0.1693 mL 0.8466 mL 1.6932 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (4.23 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.23 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

Cardiotoxin Analog (CTX) IV (6-12) (TFA)

发表于由

Cardiotoxin Analog (CTX) IV (6-12) (TFA); 纯度: 98.58%

Cardiotoxin Analog (CTX) IV (6-12) (TFA) 是心脏毒素类似物IV。Cardiotoxin analogues IV是从台湾眼镜蛇的毒液中分离出来，是一种独特的蛇毒心脏毒素。

Cardiotoxin Analog (CTX) IV (6-12) (TFA)amp;;

Cardiotoxin Analog (CTX) IV (6-12) (TFA) Chemical Structure

规格 价格 是否有货 数量
1 mg ￥700 In-stock
5 mg ￥2000 In-stock
10 mg ￥3600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Cardiotoxin Analog (CTX) IV (6-12) (TFA) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Cardiotoxin Analog (CTX) IV (6-12) (TFA) is a part peptide of Cardiotoxin Analog (CTX) IV. Cardiotoxin analogues IV isolated from the venom of Taiwan Cobra. CTX IV is an unique snake venom cardiotoxin[1].

分子量

1013.16

Formula

C50H71F3N10O9
Sequence

Leu-Ile-Pro-Pro-Phe-Trp-Lys-NH2
Sequence Shortening

LIPPFWK-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 50 mg/mL (49.35 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9870 mL 4.9351 mL 9.8701 mL
5 mM 0.1974 mL 0.9870 mL 1.9740 mL
10 mM 0.0987 mL 0.4935 mL 0.9870 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Kaneda N, et al. The amino acid sequence of cardiotoxin-analogue IV from the venom of Naja naja atra. FEBS Lett. 1976 Nov;70(1):217-22.

荧光染料Hoechst 33258 analog 3

发表于由

荧光染料Dye Reagents Hoechst 33258 analog 3; 纯度: 99.89%

Hoechst 33258 analog 3 是Hoechst染料的一种，能结合DNA，发蓝色荧光。

Hoechst 33258 analog 3

Hoechst 33258 analog 3 Chemical Structure

CAS No. : 23554-98-5

规格 价格 是否有货 数量
5 mg ￥3500 In-stock
10 mg ￥5000 In-stock
50 mg ￥15000 In-stock
100 mg ￥21000 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33258 analog 3 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33258 analog 3 are part of a family of blue fluorescent dyes used to stain DNA. IC50 Value: Target: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.

分子量

422.52

Formula

C26H26N6
CAS 号

23554-98-5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 3.57 mg/mL (8.45 mM; ultrasonic and warming and heat to 70°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3668 mL 11.8338 mL 23.6675 mL
5 mM 0.4734 mL 2.3668 mL 4.7335 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4′,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.

    [2]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505.

    [3]. a b c “Hoechst Stains”. Invitrogren (Molecular Probes).

荧光染料Hoechst 33342 analog 2

发表于由

荧光染料Dye Reagents Hoechst 33342 analog 2;

Hoechst 33342 analog 2 是 Hoechst 33342 的一种类似物, Hoechst 33342 结合到 DNA 小沟，用于细胞 DNA 荧光染色。

Hoechst 33342 analog 2

Hoechst 33342 analog 2 Chemical Structure

CAS No. : 106050-84-4

规格 价格 是否有货
10;mM;*;1 mL in DMSO ￥4238 询问价格 货期
5 mg ￥3500 询问价格 货期
10 mg ￥5000 询问价格 货期
50 mg ￥15000 询问价格 货期

* Please select Quantity before adding items.

Hoechst 33342 analog 2 的其他形式现货产品:

Hoechst 33342 Hoechst 33342 trihydrochloride Hoechst 33342 analog Hoechst 33342 analog 2 trihydrochloride

生物活性

Hoechst 33342 analog 2 is an anglog of Hoechst 33342, which is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

分子量

550.39

Formula

C25H23IN6O
CAS 号

106050-84-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 57 mg/mL (103.56 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8169 mL 9.0845 mL 18.1689 mL
5 mM 0.3634 mL 1.8169 mL 3.6338 mL
10 mM 0.1817 mL 0.9084 mL 1.8169 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

荧光染料Hoechst 33258 analog 6

发表于由

荧光染料Dye Reagents Hoechst 33258 analog 6; 纯度: 98.73%

Hoechst 33258类似物6是Hoechst染料中的一种，Hoechst 33258能用于DNA定量的细胞染料。

Hoechst 33258 analog 6

Hoechst 33258 analog 6 Chemical Structure

CAS No. : 129244-66-2

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥4960 In-stock
5 mg ￥4133 In-stock
10 mg ￥5000 In-stock
50 mg ￥15000 In-stock
100 mg ￥21000 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33258 analog 6 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33258 analog 6 is a anglog of Hoechst stains(Hoechst 33258), which are part of a family of blue fluorescent dyes used to stain DNA. IC50 Value: Target: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.

分子量

536.71

Formula

C33H40N6O
CAS 号

129244-66-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 25 mg/mL (46.58 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8632 mL 9.3160 mL 18.6320 mL
5 mM 0.3726 mL 1.8632 mL 3.7264 mL
10 mM 0.1863 mL 0.9316 mL 1.8632 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.66 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (4.66 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.66 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505.

    [2]. a b c “Hoechst Stains”. Invitrogren (Molecular Probes).

    [3]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4′,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.

荧光染料Hoechst 33258 analog 5

发表于由

荧光染料Dye Reagents Hoechst 33258 analog 5; 纯度: 99.44%

Hoechst 33258类似物5是Hoechst染料中的一种，可结合DNA，发蓝色荧光。Hoechst 33258是可用于DNA定量的细胞染色剂。

Hoechst 33258 analog 5

Hoechst 33258 analog 5 Chemical Structure

CAS No. : 23491-55-6

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥4240 In-stock
5 mg ￥3500 In-stock
10 mg ￥5000 In-stock
50 mg ￥15000 In-stock
100 mg ￥21000 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33258 analog 5 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33258 analog 5 is a analog of Hoechst stains, which are part of a family of blue fluorescent dyes used to stain DNA. IC50 Value: Target: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.

分子量

458.56

Formula

C29H26N6
CAS 号

23491-55-6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 33.33 mg/mL (72.68 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1807 mL 10.9037 mL 21.8074 mL
5 mM 0.4361 mL 2.1807 mL 4.3615 mL
10 mM 0.2181 mL 1.0904 mL 2.1807 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (5.45 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.45 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.45 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.45 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505.

    [2]. a b c “Hoechst Stains”. Invitrogren (Molecular Probes).

    [3]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4′,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.

Hoechst 33342 analog 2 trihydrochloride

发表于由

Hoechst 33342 analog 2 trihydrochloride; 纯度: 98.13%

Hoechst 33342 analog 2 trihydrochloride 是 Hoechst 33342 的一种类似物, Hoechst 33342 结合到 DNA 小沟，用于细胞 DNA 荧光染色。

Hoechst 33342 analog 2 trihydrochlorideamp;;

Hoechst 33342 analog 2 trihydrochloride Chemical Structure

CAS No. : 155815-98-8

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ￥5080 In-stock
5 mg ￥3500 In-stock
10 mg ￥5000 In-stock
50 mg ￥15000 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33342 analog 2 trihydrochloride 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33342 analog 2 trihydrochloride is a anglog of Hoechst 33342. Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

分子量

659.78

Formula

C25H26Cl3IN6O
CAS 号

155815-98-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

H2O : 20 mg/mL (30.31 mM; Need ultrasonic)

DMSO : 4 mg/mL (6.06 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5157 mL 7.5783 mL 15.1566 mL
5 mM 0.3031 mL 1.5157 mL 3.0313 mL
10 mM 0.1516 mL 0.7578 mL 1.5157 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.