Arbutin (β-Arbutin) is a competitive inhibitor of tyrosinase in melanocytes, with Kiapp values of 1.42 mM for monophenolase; 0.9 mM for diphenolase. Arbutin is also used as depigmenting agents[1]. Arbutin is a natural polyphenol isolated from the bearberry plant Arctostaphylos uvaursi, possesses with anti-oxidant, anti-inflammatory and anti-tumor properties[2][3].
IC50 Target
Tyrosinase[1]
体外研究 (In Vitro)
Arbutin (0.3-5.4 mM; 24 hours, 48 hours, 72 hours; B16 murine melanoma cells) inhibites the viability of B16 murine melanoma cells in a time-and dose-dependent manner[2]. Arbutin (1.4-5.4 mM; 24 hours) increases the apoptosis rate of B16 murine melanoma cell of treatment at a dose of 5.4 mM[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[2]
Cell Line:
B16 murine melanoma cells
Concentration:
0.3 mM, 0.7 mM, 1.4 mM, 2.1 mM, 2.9 mM, 3.6 mM, 5.4 mM
Incubation Time:
24 hours, 48 hours or 72 hours
Result:
Inhibited the viability of B16 murine melanoma cells in a time- and dose-dependent manner.
Apoptosis Analysis[2]
Cell Line:
B16 murine melanoma cells
Concentration:
1.4 mM, 2.9 mM, 5.4 mM
Incubation Time:
24 hours
Result:
Caused apoptosis in 19.3% of the cells.
体内研究 (In Vivo)
Arbutin (50 mg/kg, 100 mg/kg; oral administration; every day; for 17 days; male C57BL/6 mice) pretreatment exhibits markedly protective effects on ISO-induced cardiac hypertrophy in mice[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Male C57BL/6 mice ( 20-25 g)[3]
Dosage:
50 mg/kg, 100 mg/kg
Administration:
Oral administration; every day; for 17 days
Result:
Ameliorated the ISO-induced myocardial injury.
分子量
272.25
Formula
C12H16O7
CAS 号
497-76-7
中文名称
熊果苷;熊果酚甙;熊果素;熊果叶甙;楊梅葉甘
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Garcia-Jimenez A, et al. Action of tyrosinase on alpha and beta-arbutin: A kinetic study. PLoS One. 2017 May 11;12(5):e0177330.
[2]. Jiang L, et al. Investigation of the pro-apoptotic effects of arbutin and its acetylated derivative on murinemelanoma cells. Int J Mol Med. 2018 Feb;41(2):1048-1054.
[3]. Nalban N, et al. Arbutin Attenuates Isoproterenol-Induced Cardiac Hypertrophy by Inhibiting TLR-4/NF-κB Pathway in Mice. Cardiovasc Toxicol. 2019 Sep 4.