标签归档：asp

Gly-Arg-Gly-Asp-Ser TFA

发表于由

Gly-Arg-Gly-Asp-Ser TFA;

Gly-Arg-Gly-Asp-Ser (TFA) 是一种五肽，构成了糖蛋白，骨桥蛋白与细胞的结合域。Gly-Arg-Gly-Asp-Ser (TFA) 与整合素整联蛋白受体 (integrin receptor) αvβ3 和 αvβ5 结合，IC50 估算值为 ∼5 和 ∼6.5 μM。

Gly-Arg-Gly-Asp-Ser TFAamp;;

Gly-Arg-Gly-Asp-Ser TFA Chemical Structure

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Gly-Arg-Gly-Asp-Ser TFA 的其他形式现货产品:

Gly-Arg-Gly-Asp-Ser

生物活性

Gly-Arg-Gly-Asp-Ser (TFA) is a pentapeptide that forms the cell-binding domain of a glycoprotein, osteopontin[1]. Gly-Arg-Gly-Asp-Ser binds to integrin receptors αvβ3 and αvβ5 with estimated IC50 of ∼5 and ∼6.5 μM[2].

IC50 Target

IC50: 5 μM (αvβ3), 6.5 μM (αvβ5)[2]
分子量

604.49

Formula

C19H31F3N8O11
Sequence

Gly-Arg-Gly-Asp-Ser
Sequence Shortening

GRGDS
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Kim GH, et al. Evaluation of Osteoblast-Like Cell Viability and Differentiation on the Gly-Arg-Gly-Asp-Ser Peptide Immobilized Titanium Dioxide Nanotube via Chemical Grafting. J Nanosci Nanotechnol. 2016 Feb;16(2):1396-9.

    [2]. Bernhagen D, et al. High-Affinity RGD-Knottin Peptide as a New Tool for Rapid Evaluation of the Binding Strength of Unlabeled RGD-Peptides to αvβ3, αvβ5, and α5β1 Integrin Receptors. Anal Chem. 2017 Jun 6;89(11):5991-5997.

[Asp5]-Oxytocin

发表于由

[Asp5]-Oxytocin;

[Asp5]-Oxytocin 是第一个具有重要生物活性的 5 位 (5-position)-神经垂体激素类似物。

[Asp5]-Oxytocinamp;;

[Asp5]-Oxytocin Chemical Structure

CAS No. : 65907-78-0

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生物活性

[Asp5]-Oxytocin is the first 5-position neurohypophyseal hormone analogue possessing significant biological activity[1].

体外研究
(In Vitro)

[Asp5]-Oxytocin retains not only a high affinity for the uterotonic receptor, but, more importantly, it exhibits an intrinsic activity identical with that of oxytocin under various experimental conditions[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1008.17

Formula

C43H65N11O13S2
CAS 号

65907-78-0
Sequence Shortening

CYIQDCPLG-NH2 (Disulfide bridge:Cys1-Cys6)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Walter R, et al. [5-Aspartic acid]-oxytocin: first 5-position neurohypophyseal hormone analogue possessing significant biological activity. J Am Chem Soc. 1978;100(3):792-793.

Fmoc-Asp(OtBu)-Thr(psi(Me,Me)pro)-OH

发表于由

Fmoc-Asp(OtBu)-Thr(psi(Me,Me)pro)-OH;

Fmoc-Asp(OtBu)-Thr(psi(Me,Me)pro)-OH 是一种二肽。

Fmoc-Asp(OtBu)-Thr(psi(Me,Me)pro)-OHamp;;

Fmoc-Asp(OtBu)-Thr(psi(Me,Me)pro)-OH Chemical Structure

CAS No. : 920519-32-0

规格 是否有货
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生物活性

Fmoc-Asp(OtBu)-Thr(psi(Me,Me)pro)-OH is a dipeptide.

分子量

552.62

Formula

C30H36N2O8
CAS 号

920519-32-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Fmoc-Asp(OtBu)-Ser(psi(Me,Me)pro)-OH

发表于由

Fmoc-Asp(OtBu)-Ser(psi(Me,Me)pro)-OH;

Fmoc-Asp(OtBu)-Ser(psi(Me,Me)pro)-OH 是一种二肽。

Fmoc-Asp(OtBu)-Ser(psi(Me,Me)pro)-OHamp;;

Fmoc-Asp(OtBu)-Ser(psi(Me,Me)pro)-OH Chemical Structure

CAS No. : 955048-92-7

规格 是否有货
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生物活性

Fmoc-Asp(OtBu)-Ser(psi(Me,Me)pro)-OH is a dipeptide.

分子量

538.59

Formula

C29H34N2O8
CAS 号

955048-92-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

ASP5286

发表于由

ASP5286;

ASP5286 是一种新型的非免疫 cyclophilin 抑制剂，用于治疗 HCV。

ASP5286amp;;

ASP5286 Chemical Structure

CAS No. : 935735-70-9

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生物活性

ASP5286 is a novel non-immunosuppressive cyclophilin inhibitor for the treatment of HCV.

分子量

1234.61

Formula

C62H111N11O14
CAS 号

935735-70-9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Makino T, et al. Discovery of ASP5286: A novel non-immunosuppressive cyclophilin inhibitor for the treatment of HCV. Bioorg Med Chem Lett. 2020 Aug 15;30(16):127308.

Asp-Asp-Asp-Asp-Asp

发表于由

Asp-Asp-Asp-Asp-Asp;

Asp-Asp-Asp-Asp-Asp 是由 5 个天冬氨酸残基组成的多肽。

Asp-Asp-Asp-Asp-Aspamp;;

Asp-Asp-Asp-Asp-Asp Chemical Structure

CAS No. : 124219-00-7

规格 价格 是否有货
1 mg ￥500 询问价格 货期
5 mg ￥1000 询问价格 货期
10 mg ￥1400 询问价格 货期

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生物活性

Asp-Asp-Asp-Asp-Asp is a peptide consists of 5 Asp.

分子量

593.45

Formula

C20H27N5O16
CAS 号

124219-00-7
Sequence

Asp-Asp-Asp-Asp-Asp
Sequence Shortening

DDDDD
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

N-Acetyl-Ser-Asp-Lys-Pro(Synonyms: Ac-SDKP)

发表于由

N-Acetyl-Ser-Asp-Lys-Pro;(Synonyms: Ac-SDKP)

N-Acetyl-Ser-Asp-Lys-Pro 是来自骨髓的内源性四肽，是 ACE 的N-末端位点的特异性底物。

N-Acetyl-Ser-Asp-Lys-Proamp;;(Synonyms: Ac-SDKP)

N-Acetyl-Ser-Asp-Lys-Pro Chemical Structure

CAS No. : 127103-11-1

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N-Acetyl-Ser-Asp-Lys-Pro 的其他形式现货产品:

N-Acetyl-Ser-Asp-Lys-Pro acetate

生物活性

N-Acetyl-Ser-Asp-Lys-Pro, an endogenous tetrapeptide secreted by bone marrow, is a specific substrate for the N-terminal site of ACE.

体外研究
(In Vitro)

N-Acetyl-Ser-Asp-Lys-Pro is degraded specifically by ACE, and its plasma level rises substantially during ACE inhibitor therapy. Flow cytometry of rat cardiac fibroblasts treated with N-Acetyl-Ser-Asp-Lys-Pro shows significant inhibition of the progression of cells from G0/G1 phase to S phase of the cell cycle. Moreover, phosphorylation and nuclear translocation of Smad2 is decreased in cardiac fibroblasts treated with N-Acetyl-Ser-Asp-Lys-Pro[1]. N-acetyl-seryl-aspartyl-lysyl-proline appears to exert this function by blocking the action of a stem cell-specific proliferation stimulator and acts selectively on quiescent progenitors[2]. N-Acetyl-Ser-Asp-Lys-Pro inhibits collagenase expression and activation is associated with increased expression of TIMP-1 and TIMP-2. N-Acetyl-Ser-Asp-Lys-Pro normalizes the IL-1β-mediated increase in MMP-2 and MMP-9 activities and MMP-13 expression[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

N-Acetyl-Ser-Asp-Lys-Pro prevents hypertension-induced inflammatory cell infiltration, collagen deposition, nephrin downregulation and albuminuria, which could lead to renoprotection in hypertensive mice[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

487.50

Formula

C20H33N5O9
CAS 号

127103-11-1
Sequence

Ac-Ser-Asp-Lys-Pro
Sequence Shortening

Ac-SDKP
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Rousseau A, et al. The hemoregulatory peptide N-acetyl-Ser-Asp-Lys-Pro is a natural and specificsubstrate of the N-terminal active site of human angiotensin-converting enzyme. J Biol Chem. 1995 Feb 24;270(8):3656-61.

    [2]. Pokharel S, et al. N-acetyl-Ser-Asp-Lys-Pro inhibits phosphorylation of Smad2 in cardiac fibroblasts. Hypertension. 2002 Aug;40(2):155-61.

    [3]. Rhaleb NE, et al. N-acetyl-Ser-Asp-Lys-Pro inhibits interleukin-1β-mediated matrix metalloproteinase activation in cardiac fibroblasts. Pflugers Arch. 2013 Oct;465(10):1487-95.

    [4]. Rhaleb NE, et al. Renal protective effects of N-acetyl-Ser-Asp-Lys-Pro in deoxycorticosterone acetate-salt hypertensive mice. J Hypertens. 2011 Feb;29(2):330-8.

FLAG peptide

发表于由

FLAG peptide; 纯度: 99.21%

FLAG peptide是一种八个氨基酸组成的多肽 (Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys)，具有肠激酶切割位点。FLAG peptide用于抗体介导的重组蛋白的识别和纯化。

FLAG peptideamp;;

FLAG peptide Chemical Structure

CAS No. : 98849-88-8

规格 价格 是否有货 数量
1 mg ￥500 In-stock
5 mg ￥900 In-stock
10 mg ￥1300 In-stock
25 mg ￥2390 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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FLAG peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library

生物活性

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

体外研究
(In Vitro)

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure[1]. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1012.97

Formula

C41H60N10O20
CAS 号

98849-88-8
Sequence

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Sequence Shortening

DYKDDDDK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light, stored under nitrogen

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (98.72 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9872 mL 4.9360 mL 9.8720 mL
5 mM 0.1974 mL 0.9872 mL 1.9744 mL
10 mM 0.0987 mL 0.4936 mL 0.9872 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65.

    [2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.

Arg-Gly-Asp-Ser(Synonyms: RGDS peptide Fibronectin tetrapeptide)

发表于由

Arg-Gly-Asp-Ser;(Synonyms: RGDS peptide; Fibronectin tetrapeptide) 纯度: 99.76%

Arg-Gly-Asp-Ser 是整联蛋白结合序列，抑制整联蛋白受体 (integrin receptor) 功能。Arg-Gly-Asp-Ser 直接并特异性地结合 pro-caspase-8，pro-caspase-9 和 pro-caspase-3，但不与 pro-caspase-1 结合。

Arg-Gly-Asp-Seramp;;(Synonyms: RGDS peptide; Fibronectin tetrapeptide)

Arg-Gly-Asp-Ser Chemical Structure

CAS No. : 91037-65-9

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥610 In-stock
10 mg ￥550 In-stock
50 mg ￥1750 In-stock
100 mg ￥3150 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

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Arg-Gly-Asp-Ser 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Peptidomimetic Library
  • Cytoskeleton Compound Library
  • Targeted Diversity Library
  • Peptide Library

生物活性

Arg-Gly-Asp-Ser is an integrin binding sequence that inhibits integrin receptor function. Arg-Gly-Asp-Ser directly and specifically bind pro-caspase-8, pro-caspase-9 and pro-caspase-3, while it does not bind pro-caspase-1.

体外研究
(In Vitro)

The Arg-Gly-Asp-Ser-modified surface causes up-regulation of αvβ3 integrin. Attachment to the Arg-Gly-Asp-Ser-treated membrane completely abolishes apoptosis induced by staurosporine, the Ca2+·Pi ion pair, and sodium nitroprusside. Arg-Gly-Asp-Ser-dependent resistance to apoptosis is eliminated, when the activity of the phosphatidylinositol 3-kinase pathway is inhibited[1]. Arg-Gly-Asp-Ser interacts with survivin, as well as with procaspase-3, -8 and -9. Arg-Gly-Asp-Ser-peptide binding to survivin is found to be specific, at high affinity (Kd 27.5 μM) and locates at the survivin C-terminus. Arg-Gly-Asp-Ser-survivin interaction appears to play a key role, since Arg-Gly-Asp-Ser lost its anti-mitogenic effect in survivin-deprived cells with a specific siRNA[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Arg-Gly-Asp-Ser (2.5 or 5 mg/kg, 1 h before LPS) significantly inhibits LPS-induced MMP-9 activity in BAL fluid 4 h post-LPS. Arg-Gly-Asp-Ser (1, 2.5 or 5 mg/kg, i.p.) administers 1 h before LPS inhibited LPS-induced increases in TNF-α and MIP-2 levels in BAL fluid at 4 h post-LPS[2]. Arg-Gly-Asp-Ser peptide significantly reduces tumor necrosis factor (TNF)-α and macrophage inflammatory protein (MIP)-2 production, and decreases myeloperoxidase (MPO) and NF-κB activity[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

433.42

Formula

C15H27N7O8
CAS 号

91037-65-9
Sequence

Arg-Gly-Asp-Ser
Sequence Shortening

RGDS
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : ≥ 55 mg/mL (126.90 mM)

H2O : ≥ 25 mg/mL (57.68 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3072 mL 11.5362 mL 23.0723 mL
5 mM 0.4614 mL 2.3072 mL 4.6145 mL
10 mM 0.2307 mL 1.1536 mL 2.3072 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Grigoriou V, et al. Apoptosis and survival of osteoblast-like cells are regulated by surface attachment. J Biol Chem. 2005 Jan 21;280(3):1733-9.

    [2]. Moon C, et al. Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. Respir Res. 2009 Mar 9;10:18.

    [3]. Yin X, et al. Synthetic RGDS peptide attenuated lipopolysaccharide/D-galactosamine-induced fulminant hepatic failure in mice. J Gastroenterol Hepatol. 2014 Jun;29(6):1308-15.

    [4]. Aguzzi MS, et al. Intracellular targets of RGDS peptide in melanoma cells. Mol Cancer. 2010 Apr 22;9:84.
Cell Assay
[1]

Cell death is measured using the MTT analysis. This assay is based on the ability of mitochondrial dehydrogenases to oxidize thiazolyl blue (MTT), a tetrazolium salt (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenylterazolium bromide), to an insoluble blue formazan product. The cells are incubated with the MTT reagent (120 μg/mL) at 37°C for 2 h. After the supernatant is removed, 400 μL of 0.04mol/LHCl in isopropanol is added to each well, and the optical density of the solution is read at 590 nm in an enzyme-linked immunosorbent assay plate reader. As the generation of the blue product is proportional to the dehydrogenase activity, a decrease in the absorbance at 590 nm provides a direct measurement of the number of viable cells. To determine the contribution of the PI3K pathway to inhibition of apoptosis, some cell populations are pretreated with 50 μM LY294002, a PI3K inhibitor. Following this pretreatment, cell death is determined as described above.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Mice pharyngeal aspiration is performed as described. Animals are anesthetized with a mixture of ketamine and xylazine (45 mg/kg and 8 mg/kg, i.p., respectively). Test solution (30 μL) containing LPS (1.5 mg/kg) is placed posterior in the throat and aspirated into the lungs. Control mice are administrated sterile saline (0.9% NaCl). Animals are administered with Arg-Gly-Asp-Ser or RGES peptide (1, 2.5 or 5 mg/kg, i.p.) once one hour before LPS treatment and sacrificed 4 h post-LPS. Animals are also administered Arg-Gly-Asp-Ser or RGES peptide (5 mg/kg, i.p.) once at different time points (1 h before or 2 h after LPS treatment) and sacrificed 24 h post-LPS. In addition, animals are administered with αvβ3-blocking mAbs, anti-αv, or anti-β3 (5 mg/kg, i.p.) once 1 h before and sacrificed 4 h post-LPS. Animals administered with these mAbs 2 h after LPS treatment are sacrificed 24 h post-LPS

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Grigoriou V, et al. Apoptosis and survival of osteoblast-like cells are regulated by surface attachment. J Biol Chem. 2005 Jan 21;280(3):1733-9.

    [2]. Moon C, et al. Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. Respir Res. 2009 Mar 9;10:18.

    [3]. Yin X, et al. Synthetic RGDS peptide attenuated lipopolysaccharide/D-galactosamine-induced fulminant hepatic failure in mice. J Gastroenterol Hepatol. 2014 Jun;29(6):1308-15.

    [4]. Aguzzi MS, et al. Intracellular targets of RGDS peptide in melanoma cells. Mol Cancer. 2010 Apr 22;9:84.

4-Di-16-ASP(Synonyms: DiA)

发表于由

4-Di-16-ASP;(Synonyms: DiA)

4-Di-16-ASP 是一种膜染料。4-Di-16-ASP 是一种亲脂性氨基苯乙烯基探针，具有宽发射光谱（可以用绿色、橙色甚至红色滤光片检测）。常用于神经元膜追踪 (它比 DiO 扩散得更快)。

4-Di-16-ASPamp;;(Synonyms: DiA)

4-Di-16-ASP Chemical Structure

CAS No. : 114041-00-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

4-Di-16-ASP is a green fluorescent membrane dye. 4-Di-16-ASP is a lipophilic aminostyryl probe with a broad emission spectrum (can be detected with green, orange or even red filters). It is commonly used for neuronal membrane tracing (it diffuses faster than DiO)[1].

分子量

787.04

Formula

C46H79IN2
CAS 号

114041-00-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Lucas McDonald, et al. An NIR Emitting Styryl Dye with Large Stokes Shift to Enable co-Staining Study on Zebrafish Neuromast Hair Cells. Bioorg Chem. 2019 Aug;89:103040.