荧光染料Brilliant Blue R250(Synonyms: Brilliant Blue R)

荧光染料Dye Reagents Brilliant Blue R250;(Synonyms: Brilliant Blue R)

Brilliant blue R250 (Brilliant Blue R) 是一种阴离子染料,是检测 SDS-PAGE 凝胶中溶解的蛋白质最常用的染色剂。

Brilliant Blue R250(Synonyms: Brilliant Blue R)

Brilliant Blue R250 Chemical Structure

CAS No. : 6104-59-2

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels[1].

分子量

825.97

Formula

C45H44N3NaO7S2

CAS 号

6104-59-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

参考文献
  • [1]. Krause RGE, et al. Crystal violet stains proteins in SDS-PAGE gels and zymograms. Anal Biochem. 2019;566:107-115.

Brilliant Blue FCF(Synonyms: Acid Blue 9 FD&C Blue No. 1 E133)

Brilliant Blue FCF;(Synonyms: Acid Blue 9; FDC Blue No. 1; E133) 纯度: ge;98.0%

Brilliant Blue FCF是一种芳香族烃类, 是从石油中生产的合成染料, 用于食品和其它物质的着色剂, 溶液最大吸收在628 nm。

Brilliant Blue FCFamp;;(Synonyms: Acid Blue 9;  FDamp;C Blue No. 1;  E133)

Brilliant Blue FCF Chemical Structure

CAS No. : 3844-45-9

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ¥500 In-stock
500 mg ¥400 In-stock
1 g ¥500 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

Brilliant Blue FCF 相关产品

bull;相关化合物库:

  • Food Additive Library
  • Inactive Ingredient Library
  • Bioactive Compound Library Plus

生物活性

Brilliant Blue FCF has the appearance of a reddish-blue powder. It is soluble in water, and the solution has a maximum absorption at about 628 nanometers. It is a synthetic dye produced using aromatic hydrocarbons from petroleum, is a colorant for foods and other substances.

分子量

792.85

Formula

C37H34N2Na2O9S3

CAS 号

3844-45-9

中文名称

食用色素亮蓝;罂红二钠盐;亮蓝 FCF;酸性亮蓝 FCF

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

H2O : 33.33 mg/mL (42.04 mM; ultrasonic and adjust pH to 11 with NaOH)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2613 mL 6.3064 mL 12.6127 mL
5 mM 0.2523 mL 1.2613 mL 2.5225 mL
10 mM 0.1261 mL 0.6306 mL 1.2613 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

荧光染料Brilliant Black BN(Synonyms: E 151)

荧光染料Dye Reagents Brilliant Black BN;(Synonyms: E 151) 纯度: ge;98.0%

Brilliant black BN (E151) 是一种偶氮染料 dye 和一种食品着色剂。Brilliant black BN (E151) 是一种很有前途的抗病毒试剂 (antiviral agent ),通过抑制 EV71 与其细胞脱衣因子亲环素a的相互作用来对抗 EV71 感染。Brilliant black BN (E151) 有潜力用于传染性疾病的相关研究。

Brilliant Black BNamp;;(Synonyms: E 151)

Brilliant Black BN Chemical Structure

CAS No. : 2519-30-4

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1490 In-stock
25 mg ¥1350 In-stock
100 mg ¥3950 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Brilliant Black BN 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Antiviral Compound Library

生物活性

Brilliant black BN (E151) is an azo dye and a food colorant[1]. Brilliant black BN is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. Brilliant black BN has the potential for the investigation of contagious disease[2].

IC50 Target

IC50: 2.39 µM- 28.12 µM (various strains of EV71)[2]

体外研究
(In Vitro)

Brilliant black BN (E151) inhibits the interaction between EV71 and its cellular uncoating factor cyclophilin A [1].
Brilliant black BN (E151) inhibited the infection EV71 -GFP at a concentration of 300 µM as evidenced from the reduced GFP signals. Brilliant black BN (E151)exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells, exhibits an IC50 value of 10.1 µM[1].
Brilliant black BN (E151) is able to inhibit all tested 28 EV71, CVA16 and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye E151 for various strains of EV71 ranged from 2.39 µM to 28.12 µM ,whereas its 50% cytotoxic concentration is 1870 µM [1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Brilliant black BN (E151)(intraperitoneal injection; 200 mg/kg; 14 days)exhibits mild illness with clinical scores less than 3 and subsequently recovered when compared with PBS-group.All E151 270 treated mice are completely protected throughout the experiment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 14-days old AG129 mice with EV71[1]
Dosage: 200 mg/kg
Administration: Intraperitoneal injection
Result: Had a Protective eddect on EV71 challenged AG129 mice by dye E151.

分子量

867.68

Formula

C28H17N5Na4O14S4

CAS 号

2519-30-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

DMSO : 125 mg/mL (144.06 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1525 mL 5.7625 mL 11.5250 mL
5 mM 0.2305 mL 1.1525 mL 2.3050 mL
10 mM 0.1152 mL 0.5762 mL 1.1525 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Lang W, et al. Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench.J Environ Manage. 2014 Jan;132:155-64.

    [2]. Meng T, et al. In Vitro and In Vivo Inhibition of the Infectivity of Human Enterovirus 71 by a Sulfonated Food Azo Dye, Brilliant Black BN.J Virol. 2019 Aug 13;93(17).

Brilliant blue G-250

生化分析试剂 Biochemical Assay Reagents
Brilliant blue G-250;

Brilliant Blue G-250 是一种常用于 SDS-PAGE 分离蛋白质可视化的染料,染色过程简单,定量高。在 Bradford 蛋白质分析中,由于 Brilliant Blue G-250 与蛋白质结合,蛋白质浓度由 595 nm 处的吸光度确定。Brilliant Blue G-250 是一种安全、高选择性的 P2×7R 拮抗剂,有望使 NLRP3炎症体失活。

Brilliant blue G-250

Brilliant blue G-250 Chemical Structure

CAS No. : 6104-58-1

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome[1][2][3].

分子量

854.02

Formula

C47H48N3NaO7S2

CAS 号

6104-58-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

参考文献
  • [1]. Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009;(30):1431. Published 2009 Aug 3.

    [2]. Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82(11):4325-4328.

    [3]. Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723.