Calcein-AM(Synonyms: 钙黄绿素-AM Calcein acetoxymethyl ester)

Calcein-AM;(Synonyms: 钙黄绿素-AM; Calcein acetoxymethyl ester) 纯度: ge;96.0%

Calcein-AM是用于测定细胞活力的可以渗透细胞的荧光染料。

Calcein-AMamp;;(Synonyms: 钙黄绿素-AM; Calcein acetoxymethyl ester)

Calcein-AM Chemical Structure

CAS No. : 148504-34-1

规格 价格 是否有货 数量
100 μg(2 mg/mL * 50 μL in DMSO) ¥1300 In-stock
500 μg(2 mg/mL * 250 μL in DMSO) ¥4750 In-stock

* Please select Quantity before adding items.

生物活性

Calcein-AM is cell-permeable fluorescent dye used to determine the cell viability.

体外研究
(In Vitro)

The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this microtest. Quantitation of killing and kinetic analysis is readily performed with the test system[1]. Calcein-AM is pH independent, better retained and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader[2]. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane. From dying or damaged cells with compromised membrane integrity or from cells expressing multidrug resistance protein (MRP), unhydrolyzed substrates and their fluorescent products are rapidly extruded from cells. The calcein-AM assay has been used to assess the cell viability, cytotoxicity and tp quantitate apoptosis[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

994.86

Formula

C46H46N2O23

CAS 号

148504-34-1

中文名称

钙黄绿素-AM

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

参考文献
  • [1]. Wang XM, et al. A new microcellular cytotoxicity test based on calcein-AM release. Hum Immunol. 1993 Aug;37(4):264-70.

    [2]. Braut-Boucher F, et al. A non-isotopic, highly sensitive, fluorimetric, cell-cell adhesion microplate assay using calceinAM-labeled lymphocytes. J Immunol Methods. 1995 Jan 13;178(1):41-51.

    [3]. Bratosin D, et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viabilityand aging. Cytometry A. 2005 Jul;66(1):78-84.

Cell Assay
[1][2][3]

K562, Daudi, and Chang liver cells are labeled with calcein-AM. Calcein-AM’s excitation and emission wavelengths are 496 nm and 520 nm, respectively. The filter/mirror combination used to detect calcein-AM’s green fluorescence includes the 490-nm excitation and 520-nm emission filters with a dichroic mirror. Differences in the automatic fluorescence readings between the test and control wells determine the results[1]. A simple and sensitive cell-cell adhesion microplate assay is established using the calcein-AM. The procedure involves three steps: the labeling of lymphocytes with an adequate concentration of calcein-AM (20 μM) during a short incubation period (30 min); the adhesion of 2×105 labeled lymphocytes per well to confluent keratinocyte or fibroblast monolayers grown in microtiter plates for 90 min; and, finally, measurement of the fluorescent signal utilizing a new system of cold-light microfluorimetry[2]. Cells are incubated for 15 min in 1 mL of a 1% saponin solution in PBS buffer, pH 7.4, containing 0.05% sodium azide. After saponin permeabilization, 4×105 RBCs in suspension in PBS buffer containing 0.1% saponin and 0.05% sodium azide are incubated (37°C in the dark for 45 min) with calcein-AM to a final concentration of 5 μM, ished three times with the same PBS buffer containing 0.1% saponin and 0.05% sodium azide, and the cell viability is analyzed by flow cytometry[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Wang XM, et al. A new microcellular cytotoxicity test based on calcein-AM release. Hum Immunol. 1993 Aug;37(4):264-70.

    [2]. Braut-Boucher F, et al. A non-isotopic, highly sensitive, fluorimetric, cell-cell adhesion microplate assay using calceinAM-labeled lymphocytes. J Immunol Methods. 1995 Jan 13;178(1):41-51.

    [3]. Bratosin D, et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viabilityand aging. Cytometry A. 2005 Jul;66(1):78-84.

荧光染料Calcein tetraethyl ester(Synonyms: 钙黄绿素四乙酯 Fluorexon tetraethyl ester)

荧光染料Dye Reagents Calcein tetraethyl ester;(Synonyms: 钙黄绿素四乙酯; Fluorexon tetraethyl ester) 纯度: ge;98.0%

钙黄绿素四乙酯是一种是荧光染料, 用于生物学,因为它可以通过细胞膜进入活细胞,可用于细胞活力检测。

Calcein tetraethyl esteramp;;(Synonyms: 钙黄绿素四乙酯; Fluorexon tetraethyl ester)

Calcein tetraethyl ester Chemical Structure

CAS No. : 1170856-93-5

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1060 In-stock
10 mg ¥950 In-stock
25 mg ¥1800 In-stock
50 mg ¥2900 In-stock
100 mg ¥5200 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Calcein tetraethyl ester 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Calcein tetraethyl ester is a fluorescent dye, is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells.

分子量

734.75

Formula

C38H42N2O13

CAS 号

1170856-93-5

中文名称

钙黄绿素四乙酯;钙黄绿素四乙基酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (34.03 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3610 mL 6.8050 mL 13.6101 mL
5 mM 0.2722 mL 1.3610 mL 2.7220 mL
10 mM 0.1361 mL 0.6805 mL 1.3610 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.40 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.40 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。

荧光染料Calcein tetraethyl ester(Synonyms: 钙黄绿素四乙酯 Fluorexon tetraethyl ester)

荧光染料Dye Reagents Calcein tetraethyl ester;(Synonyms: 钙黄绿素四乙酯; Fluorexon tetraethyl ester) 纯度: ge;98.0%

钙黄绿素四乙酯是一种是荧光染料, 用于生物学,因为它可以通过细胞膜进入活细胞,可用于细胞活力检测。

Calcein tetraethyl ester(Synonyms: 钙黄绿素四乙酯; Fluorexon tetraethyl ester)

Calcein tetraethyl ester Chemical Structure

CAS No. : 1170856-93-5

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1060 In-stock
10 mg ¥950 In-stock
25 mg ¥1800 In-stock
50 mg ¥2900 In-stock
100 mg ¥5200 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Calcein tetraethyl ester 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Calcein tetraethyl ester is a fluorescent dye, is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells.

分子量

734.75

Formula

C38H42N2O13

CAS 号

1170856-93-5

中文名称

钙黄绿素四乙酯;钙黄绿素四乙基酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (34.03 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3610 mL 6.8050 mL 13.6101 mL
5 mM 0.2722 mL 1.3610 mL 2.7220 mL
10 mM 0.1361 mL 0.6805 mL 1.3610 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.40 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.40 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。

Calcein(Synonyms: 钙黄绿素 Fluorexon)

Calcein;(Synonyms: 钙黄绿素; Fluorexon)

Calcein 是一种荧光染料和自灭探针,可以作为脂囊泡渗漏的指示剂,也可作 EDTA 钙离子滴定和荧光测定钙离子的络合指示剂。

Calceinamp;;(Synonyms: 钙黄绿素; Fluorexon)

Calcein Chemical Structure

CAS No. : 1461-15-0

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥550 In-stock
100 mg ¥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Calcein 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

体外研究
(In Vitro)

Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

622.53

Formula

C30H26N2O13

CAS 号

1461-15-0

中文名称

钙黄绿素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : ≥ 100 mg/mL (160.63 mM)

0.1 M NaOH : 10 mg/mL (16.06 mM; ultrasonic and adjust pH to 11 with NaOH)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6063 mL 8.0317 mL 16.0635 mL
5 mM 0.3213 mL 1.6063 mL 3.2127 mL
10 mM 0.1606 mL 0.8032 mL 1.6063 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;2% NaHCO3 in ddH2O

    Solubility: 20 mg/mL (32.13 mM); Clear solution; Need ultrasonic and adjust pH to 6 with NaHCO3

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203.

Cell Assay
[1]

Coelomocytes suspensions (ISO and CF) are separately incubated with calcein-AM (excitation and emission wavelength: 496 nm and 516 nm, respectively) at a final concentration of 200 nM, during 30 min, at 26°C. Calcein-AM is a nonfluorescent ABC transporter substrate whose intracellular accumulation is inversely proportional to ABC transporter activity. Intracellular esterases convert calcein-AM into the fluorescent dye calcein, which is not an ABC transporter substrate, thereby accumulating the dye inside the cell. Therefore, a high fluorescence signal indicates low ABC transporter activity whereas a low fluorescence signal indicates high activity. The fluorescence of samples is measured by flow cytometer. The experiment is repeated six times in duplicates.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203.

Calcein Blue(Synonyms: 钙黄绿素蓝)

Calcein Blue;(Synonyms: 钙黄绿素蓝) 纯度: 98.66%

Calcein Blue 是一种不渗透膜的荧光染料,是一种含有亚氨基二乙酸结构的香豆素衍生物。Calcein Blue 也是一种金属荧光变色指示剂。

Calcein Blueamp;;(Synonyms: 钙黄绿素蓝)

Calcein Blue Chemical Structure

CAS No. : 54375-47-2

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥550 In-stock
25 mg ¥500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Calcein Blue 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator[1][2][3].

体外研究
(In Vitro)

The fluorescence of Calcein Blue (CB) is quenched by the Fe2+ ion in the Calcein Blue-Fe2+ complex. When dopamine is added to a solution of the Calcein Blue-Fe2+ complex, a dopamine-Fe2+ complex is formed as the result of a ligand exchange reaction between Calcein Blue and dopamine which permits the fluorescence from Calcein Blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the Calcein Blue-Fe2+ complex solution, which permits dopamine to be quantitatively determined. The Calcein Blue-Fe2+ complex shows a high selectivity for dopamine among the catecholamines and related compounds examined[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

321.28

Formula

C15H15NO7

CAS 号

54375-47-2

中文名称

钙黄绿素蓝

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 6 mg/mL (18.68 mM; Need ultrasonic and warming)

H2O : 1 mg/mL (3.11 mM; ultrasonic and warming and heat to 80°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.1125 mL 15.5627 mL 31.1255 mL
5 mM 0.6225 mL 3.1125 mL 6.2251 mL
10 mM 0.3113 mL 1.5563 mL 3.1125 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43.

    [2]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49.

    [3]. G M Huitink, et al. Methyl calcein blue and other analogues of calcein blue. Talanta. 1974 Nov;21(11):1193-202.

Cell Assay

The recommended final working concentrations is usually between 1 and 10 μM to minimize potential artifacts. Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes. A stock concentration of 5 mM 4-methylumbelliferone-8- methyliminodiacetic acid, commonly known as calcein blue is prepared in 0.1 M potassium hydroxide, and the pH is neutralized using 0.1 N hydrochloric acid. The calcein blue stock prepared is diluted to 200 μM (working stock) in DPBS and stored for further use. A concentration of 10 μM calcein blue is prepared from the working stock by diluting in DPBS. From this, 200 μL is added to a 96-well plate and the excitation/emission (Ex./Em.) maximum is determined using the micro-plate reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43.

    [2]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49.

    [3]. G M Huitink, et al. Methyl calcein blue and other analogues of calcein blue. Talanta. 1974 Nov;21(11):1193-202.